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Literature summary for 2.3.3.16 extracted from

  • Rault-Leonardon, M.; Atkinson, M.A.L.; Slaughter, C.A.; Moomaw, C.R.; Srere, P.A.
    Azotobacter vinelandii citrate synthase (1995), Biochemistry, 34, 257-263.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADH 70% inhibition at 2 mM, completely reversed by 0.17 mM 5'-AMP Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information allosteric, Hill coefficient: 1.5 Azotobacter vinelandii
0.014
-
acetyl-CoA with 0.1 M KCl Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
alignment of partial amino acid sequence Azotobacter vinelandii
48000
-
6 * 48000, SDS-PAGE Azotobacter vinelandii
249000 275000 equilibrium sedimentation Azotobacter vinelandii
330000
-
gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O Azotobacter vinelandii high activity necessary for N2 fixation citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Azotobacter vinelandii citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O high activity necessary for N2 fixation Azotobacter vinelandii citrate + CoA
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 48000, SDS-PAGE Azotobacter vinelandii

Synonyms

Synonyms Comment Organism
CS
-
Azotobacter vinelandii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
enzyme assay at Azotobacter vinelandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
111
-
oxaloacetate
-
Azotobacter vinelandii
170
-
oxaloacetate with 0.1 mM KCl Azotobacter vinelandii
174
-
acetyl-CoA with 0.1 M KCl Azotobacter vinelandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
enzyme assay at Azotobacter vinelandii

Cofactor

Cofactor Comment Organism Structure
AMP positive effector Azotobacter vinelandii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
NADH in 20 mM Tris-HCl Azotobacter vinelandii
0.66
-
NADH in 0.1 M Tris-HCl Azotobacter vinelandii