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Literature summary for 2.3.3.14 extracted from

  • Qian, J.; Khandogin, J.; West, A.H.; Cook, P.F.
    Evidence for a catalytic dyad in the active site of homocitrate synthase from Saccharomyces cerevisiae (2008), Biochemistry, 47, 6851-6858.
    View publication on PubMed
Search for more literature for 2.3.3.14

Protein Variants

Protein Variants Comment Organism
E155A mutant enzyme exhibits 1000fold lower activity than wild-type enzyme. Activity of E155A can be partially rescued by formate Saccharomyces cerevisiae
E155Q mutant enzyme exhibits 1000fold lower activity than wild-type enzyme. The kcat for E155Q decreases at high pH, similar to the wild-type enzyme, but is pH independent at low pH Saccharomyces cerevisiae
H309A inactive mutant enzyme. Slight increase in activity is observed for H309A in the presence of 300 mM imidazole, which is still 1000fold lower than that of wild type Saccharomyces cerevisiae
H309N inactive mutant enzyme Saccharomyces cerevisiae
Y320F mutant enzyme loses 25fold activity compared to that of the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 acetyl-CoA mutant enzyme E155A Saccharomyces cerevisiae
0.14
-
 acetyl-CoA wild-type enzyme Saccharomyces cerevisiae
0.14
-
 acetyl-CoA mutant enzyme Y320F Saccharomyces cerevisiae
13
-
 2-oxoglutarate mutant enzyme E155A Saccharomyces cerevisiae
33
-
 2-oxoglutarate mutant enzyme Y320F Saccharomyces cerevisiae
42
-
 2-oxoglutarate wild-type enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate a catalytic dyad comprising Glu155 and His309 acts to deprotonate the methyl group of acetyl-CoA, while Tyr320 is likely not directly involved in catalysis, but may aid in orienting the reactant and/or the catalytic dyad Saccharomyces cerevisiae 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
acetyl-coenzyme A: 2-ketoglutarate C-transferase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the kcat for E155Q decreases at high pH, similar to the wild type enzyme, but is pH independent at low pH Saccharomyces cerevisiae