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Literature summary for 2.3.3.13 extracted from

  • Yindeeyoungyeon, W.; Likitvivatanavong, S.; Palittapongarnpim, P.
    Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis (2009), BMC Microbiol., 9, 122.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the coding sequences of alpha-IPMS-2CR and alpha-IPMS-14CR are cloned into the vector pET15b for expression in Escherichia coli BL21DE3 cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
Y410F mutant, insensitive to feedback inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
acetyl-CoA alpha-IPMS-14CR Mycobacterium tuberculosis
0.035
-
alpha-ketoisovaleric acid alpha-IPMS-14CR Mycobacterium tuberculosis
0.261
-
alpha-ketoisovaleric acid alpha-IPMS-2CR Mycobacterium tuberculosis
0.568
-
acetyl-CoA alpha-IPMS-2CR Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
alpha-IPMS-2CR, determined by SDS-PAGE Mycobacterium tuberculosis
99000
-
alpha-IPMS-14CR, determined by SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis
-
(2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis H37Rv
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQB3
-
-
Mycobacterium tuberculosis H37Rv P9WQB3
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA resin Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis H37Rv (2S)-2-isopropylmalate + CoA
-
?
alpha-ketoisovaleric acid + H2O + acetyl-CoA
-
Mycobacterium tuberculosis ? + CoA
-
?
alpha-ketoisovaleric acid + H2O + acetyl-CoA
-
Mycobacterium tuberculosis H37Rv ? + CoA
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 74000, alpha-IPMS-2CR, 2 * 99000, alpha-IPMS-14CR, determined by gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
alpha-IPMS
-
Mycobacterium tuberculosis
alpha-IPMS-14CR
-
Mycobacterium tuberculosis
alpha-IPMS-14CR the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-14CR comprises 14 copies Mycobacterium tuberculosis
alpha-IPMS-2CR the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-2CR comprises two copies Mycobacterium tuberculosis
Alpha-isopropylmalate synthase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 42
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
alpha-ketoisovaleric acid alpha-IPMS-14CR Mycobacterium tuberculosis
0.61
-
acetyl-CoA alpha-IPMS-14CR Mycobacterium tuberculosis
1.17
-
alpha-ketoisovaleric acid alpha-IPMS-2CR Mycobacterium tuberculosis
2.22
-
acetyl-CoA alpha-IPMS-2CR Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
activity assay Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Mycobacterium tuberculosis