Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout-mutant of isoform IPMS1 shows an increase in valine content, but no change in leucine content. Insertion mutation for isoform IPMS2 do not show any changes in soluble amino acid content. | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete loss of activity; 10 mM, complete loss of activity | Arabidopsis thaliana | |
Leu | inhibition of both enzymes reaches a maximum of 30% to 35% around 1 mM Leu; inhibition of both enzymes reaches a maximum of 30% to 35% around 1 mM Leu | Arabidopsis thaliana | |
leucine | 1 mM, 30-35% inhibition, both isoforms IPMS1 and IPMS2 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
acetyl-CoA | - |
Arabidopsis thaliana | |
0.016 | - |
acetyl-CoA | pH 8.0, isoform IPMS2 | Arabidopsis thaliana | |
0.045 | - |
acetyl-CoA | - |
Arabidopsis thaliana | |
0.045 | - |
acetyl-CoA | pH 8.0, isoform IPMS1 | Arabidopsis thaliana | |
0.279 | - |
2-oxo-3-methylbutanoate | - |
Arabidopsis thaliana | |
0.279 | - |
2-oxo-3-methylbutanoate | pH 8.0, isoform IPMS2 | Arabidopsis thaliana | |
0.304 | - |
2-oxo-3-methylbutanoate | - |
Arabidopsis thaliana | |
0.304 | - |
2-oxo-3-methylbutanoate | pH 8.0, isoform IPMS1 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 4 mM, slight stimulation | Arabidopsis thaliana | |
Mg2+ | required, both isoforms IPMS1 and IPMS2 | Arabidopsis thaliana | |
Mg2+ | requires Mg2+ in millimolar concentrations | Arabidopsis thaliana | |
Mn2+ | after treatment with EDTA, 4 mM Mn2+ restores about 50% of the initial activity | Arabidopsis thaliana | |
Mn2+ | after tretment with EDTA, 4 mM Mn2+ restores about 50% of the initial activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62300 | - |
2 * 62300, calculated from nucleotide sequence | Arabidopsis thaliana |
63100 | - |
2 * 63100, calculated, isoform IPMS1 with His-tag | Arabidopsis thaliana |
64100 | - |
4 * 64100, calculated, isoform IPMS2 with His-tag | Arabidopsis thaliana |
64100 | - |
4 * 64100, calculated from nucleotide sequence | Arabidopsis thaliana |
124000 | - |
gel filtration | Arabidopsis thaliana |
124000 | - |
gel filtration, isoform IPMS1 | Arabidopsis thaliana |
280000 | - |
gel filtration | Arabidopsis thaliana |
280000 | - |
gel filtration, isoform IPMS2 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoform IPMS1 and isoform IPMS2 | - |
Arabidopsis thaliana | Q9C550 | - |
- |
Arabidopsis thaliana | Q9LPR4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | isoform IPMS1, 112% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 97.5% of the rate with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | 112.2% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | 97.5% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-n-pentanoate + acetyl-CoA + H2O | isoform IPMS1, 27.2% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 33.8% of the rate with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-hydroxy-2-propylsuccinic acid + CoA | - |
? | |
2-oxovalerate + acetyl-CoA + H2O | 27.2% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | ? | - |
? | |
2-oxovalerate + acetyl-CoA + H2O | 33.8% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | ? | - |
? | |
4-methylthio-2-oxopentanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | ? + CoA | at very low rate, reaction is involved in glucosinolate chain elongation | ? | |
pyruvate + acetyl-CoA + H2O | isoform IPMS1, 12.7% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 15.7% of the rate with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | 12.7% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | 15.7% of the activity with 2-oxo-3-methylbutanoate | Arabidopsis thaliana | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63100, calculated, isoform IPMS1 with His-tag | Arabidopsis thaliana |
dimer | 2 * 62300, calculated from nucleotide sequence | Arabidopsis thaliana |
tetramer | 4 * 64100, calculated, isoform IPMS2 with His-tag | Arabidopsis thaliana |
tetramer | 4 * 64100, calculated from nucleotide sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
2-isopropylmalate synthase 1, chloroplastic | - |
Arabidopsis thaliana |
2-isopropylmalate synthase 2, chloroplastic | - |
Arabidopsis thaliana |
IPMS1 | - |
Arabidopsis thaliana |
IPMS2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
acetyl-CoA | - |
Arabidopsis thaliana | |
1.9 | - |
acetyl-CoA | pH 8.0, isoform IPMS2 | Arabidopsis thaliana | |
2.2 | - |
acetyl-CoA | - |
Arabidopsis thaliana | |
2.2 | - |
acetyl-CoA | pH 8.0, isoform IPMS1 | Arabidopsis thaliana | |
2.3 | - |
2-oxo-3-methylbutanoate | - |
Arabidopsis thaliana | |
2.3 | - |
2-oxo-3-methylbutanoate | pH 8.0, isoform IPMS2 | Arabidopsis thaliana | |
2.4 | - |
2-oxo-3-methylbutanoate | - |
Arabidopsis thaliana | |
2.4 | - |
2-oxo-3-methylbutanoate | pH 8.0, isoform IPMS1 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Arabidopsis thaliana |
8.5 | - |
both isoforms IPMS1 and IPMS2 | Arabidopsis thaliana |