Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
in complex with hymeglusin, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.3-6.5), 0.2 M NaCl, and 23% (w/v) polyethylene glycol 3350 | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hymeglusin | i.e. (2E,4E,7R)-11-[(2R,3R)-3-(hydroxymethyl)-4-oxooxetan-2-yl]-3,5,7-trimethylundeca-2,4-dienoic acid, time-dependent loss of activity is observed with more than 95% inhibition achieved at extended incubation times (10 min at 75-600 nM) | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q9FD71 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA Sepharose column chromatography and Resource Q anion-exchange column chromatography | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Enterococcus faecalis | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HMG-CoA synthase | - |
Enterococcus faecalis |
mvaS | - |
Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
hymeglusin | in 100 mM Tris-HCl (pH 8.0), at 18°C | Enterococcus faecalis |