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Literature summary for 2.3.3.10 extracted from

  • Sutherlin, A.; Hedl, M.; Sanchez-Neri, B.; Burgner, J.W.; Stauffacher, C.V.; Rodwell, V.W.
    Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis (2002), J. Bacteriol., 184, 4065-4070.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.10

Activating Compound

Activating Compound Comment Organism Structure
MgCl2
-
 Gallus gallus
MgCl2 optimal activity in 2.0 mM Enterococcus faecalis

Application

Application Comment Organism
medicine
-
 Homo sapiens
medicine potential of the mevalonate pathway enzymes of enterococci as targets for antibiotics Enterococcus faecalis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Gallus gallus
cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway Streptomyces sp.
expression of recombinant wild-type cytoplasmic and Cys129 mutant enzymes Homo sapiens
mvaS gene isolated, expressed in Escherichia coli from a pET28 vector Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 acetoacetyl-CoA
-
 Gallus gallus
0.0015
-
 acetyl-CoA
-
 Blattella germanica
0.01
-
 acetoacetyl-CoA
-
 Enterococcus faecalis
0.27
-
 acetyl-CoA
-
 Gallus gallus
0.35
-
 acetyl-CoA
-
 Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gallus gallus 5737
-
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Rattus norvegicus 5737
-
cytoplasm
-
 Bos taurus 5737
-
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Mesocricetus auratus 5829
-
cytosol
-
 Blattella germanica 5829
-
mitochondrion
-
 Gallus gallus 5739
-
mitochondrion
-
 Mus musculus 5739
-
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion
-
 Sus scrofa 5739
-
mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, calculated from amino acid sequence Enterococcus faecalis
45000
-
 2 * 45000, SDS-PAGE Enterococcus faecalis
83900
-
 analytical ultracentrifugation Enterococcus faecalis
84300
-
 calculated from amino acid sequence Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus aureus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Sus scrofa
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Bos taurus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Streptococcus pneumoniae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Streptococcus pyogenes
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Mesocricetus auratus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus epidermidis
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus haemolyticus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Streptomyces sp.
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Borreliella burgdorferi
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Enterococcus faecalis isopentenyl diphosphate biosynthesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Blattella germanica
-
 cockroach
-
Borreliella burgdorferi
-
-
-
Bos taurus
-
 ox
-
Enterococcus faecalis
-
-
-
Gallus gallus
-
 avian
-
Homo sapiens
-
 human
-
Mesocricetus auratus
-
 syrian hamster, golden hamster
-
Mus musculus
-
 mouse
-
Rattus norvegicus
-
 rat
-
Staphylococcus aureus
-
-
-
Staphylococcus epidermidis
-
-
-
Staphylococcus haemolyticus
-
-
-
Streptococcus pneumoniae
-
-
-
Streptococcus pyogenes
-
-
-
Streptomyces sp.
-
-
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
recombinant enzyme Enterococcus faecalis

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Mus musculus
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus aureus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptococcus pneumoniae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptococcus pyogenes (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mesocricetus auratus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus epidermidis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus haemolyticus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptomyces sp. (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Borreliella burgdorferi (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O isopentenyl diphosphate biosynthesis Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Subunits

Subunits Comment Organism
dimer
-
 Gallus gallus
dimer
-
 Homo sapiens
dimer
-
 Bos taurus
dimer 2 * 45000, SDS-PAGE Enterococcus faecalis
dimer 2 * 42000, calculated from amino acid sequence Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
-
 Enterococcus faecalis