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Literature summary for 2.3.3.10 extracted from

  • Chun, K.Y.; Vinarov, D.A.; Miziorko, H.M.
    3-Hydroxy-3-methylglutaryl-CoA synthase: Participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate (2000), Biochemistry, 39, 14670-14681.
    View publication on PubMed
Search for more literature for 2.3.3.10

Application

Application Comment Organism
medicine deficiency of the mitochondrial isoform correlates with human metabolic disease, cytosolic isoform is potentially useful target for drugs aimed at lowering cholesterol levels Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Gallus gallus
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Homo sapiens
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00119
-
 acetoacetyl-CoA
-
 Gallus gallus
0.0078
-
 acetyl-CoA mutant D159A Gallus gallus
0.0081
-
 acetyl-CoA mutant D159A, acetyl-S-enzyme formation Gallus gallus
0.014
-
 acetyl-CoA wild-type, acetyl-CoA hydrolysis Gallus gallus
0.0227
-
 acetyl-CoA wild-type, acetyl-S-enzyme formation Gallus gallus
0.033
-
 acetyl-CoA mutant D159A, acetyl-CoA hydrolysis Gallus gallus
0.037
-
 acetyl-CoA mutant D203A Gallus gallus
0.049
-
 acetoacetyl-CoA mutant D159A Gallus gallus
0.066
-
 acetoacetyl-CoA mutant D99A Gallus gallus
0.118
-
 acetyl-CoA mutant D64N Gallus gallus
0.189
-
 acetyl-CoA mutant E63Q Gallus gallus
0.207
-
 acetyl-CoA mutant D99A Gallus gallus
0.29
-
 acetyl-CoA wild-type enzyme Gallus gallus
0.319
-
 acetyl-CoA mutant D217A Gallus gallus
0.354
-
 acetyl-CoA mutant E37Q Gallus gallus
0.358
-
 acetyl-CoA mutant D282A Gallus gallus
0.372
-
 acetyl-CoA mutant E121A Gallus gallus
0.448
-
 acetyl-CoA mutant D203A, acetyl-S-enzyme formation Gallus gallus
0.623
-
 acetyl-CoA mutant D99A, acetyl-S-enzyme formation Gallus gallus
0.677
-
 acetyl-CoA mutant D124A Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cholesterogenic isoform Gallus gallus 5737
-
cytoplasm cholesterogenic isoform Homo sapiens 5737
-
cytoplasm cholesterogenic isoform Saccharomyces cerevisiae 5737
-
cytoplasm cholesterogenic isoform Methanothermobacter thermautotrophicus 5737
-
cytoplasm cholesterogenic isoform Arabidopsis thaliana 5737
-
cytoplasm cholesterogenic isoform Borreliella burgdorferi 5737
-
cytoplasm cholesterogenic isoform Caenorhabditis elegans 5737
-
cytoplasm cholesterogenic isoform Mus musculus 5737
-
cytoplasm cholesterogenic isoform Pinus sylvestris 5737
-
cytoplasm cholesterogenic isoform Rattus norvegicus 5737
-
cytoplasm cholesterogenic isoform Schizosaccharomyces pombe 5737
-
cytoplasm cholesterogenic isoform Sus scrofa 5737
-
cytosol
-
 Mesocricetus auratus 5829
-
cytosol
-
 Blattella germanica 5829
-
cytosol
-
 Cricetulus griseus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol cholesterogenic isoform Gallus gallus 5829
-
cytosol cholesterogenic isoform Homo sapiens 5829
-
cytosol cholesterogenic isoform Saccharomyces cerevisiae 5829
-
cytosol cholesterogenic isoform Methanothermobacter thermautotrophicus 5829
-
cytosol cholesterogenic isoform Arabidopsis thaliana 5829
-
cytosol cholesterogenic isoform Borreliella burgdorferi 5829
-
cytosol cholesterogenic isoform Caenorhabditis elegans 5829
-
cytosol cholesterogenic isoform Mus musculus 5829
-
cytosol cholesterogenic isoform Pinus sylvestris 5829
-
cytosol cholesterogenic isoform Rattus norvegicus 5829
-
cytosol cholesterogenic isoform Schizosaccharomyces pombe 5829
-
cytosol cholesterogenic isoform Sus scrofa 5829
-
mitochondrion ketogenic isoform Gallus gallus 5739
-
mitochondrion ketogenic isoform Homo sapiens 5739
-
mitochondrion ketogenic isoform Saccharomyces cerevisiae 5739
-
mitochondrion ketogenic isoform Methanothermobacter thermautotrophicus 5739
-
mitochondrion ketogenic isoform Arabidopsis thaliana 5739
-
mitochondrion ketogenic isoform Borreliella burgdorferi 5739
-
mitochondrion ketogenic isoform Caenorhabditis elegans 5739
-
mitochondrion ketogenic isoform Mus musculus 5739
-
mitochondrion ketogenic isoform Pinus sylvestris 5739
-
mitochondrion ketogenic isoform Rattus norvegicus 5739
-
mitochondrion ketogenic isoform Schizosaccharomyces pombe 5739
-
mitochondrion ketogenic isoform Sus scrofa 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Methanothermobacter thermautotrophicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Mesocricetus auratus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Arabidopsis thaliana
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Borreliella burgdorferi
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Caenorhabditis elegans
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Cricetulus griseus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Pinus sylvestris
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Schizosaccharomyces pombe
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Sus scrofa
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P54873
-
-
Blattella germanica P54870 cockroach, cytosolic isoform 2
-
Blattella germanica P54961 cockroach, cytosolic isoform 1
-
Borreliella burgdorferi O51626
-
-
Caenorhabditis elegans P54871
-
-
Cricetulus griseus P13704 chinese hamster, cytosolic isoform
-
Gallus gallus
-
 avian
-
Gallus gallus P23228 chicken, cytosolic isoform
-
Homo sapiens
-
 human
-
Homo sapiens P54868 human, mitochondrial isoform
-
Homo sapiens Q01581 human, cytosolic isoform
-
Mesocricetus auratus
-
 syrian hamster, golden hamster
-
Methanothermobacter thermautotrophicus
-
-
-
Mus musculus P54869 mouse, mitochondrial isoform
-
Pinus sylvestris P93773 pine
-
Rattus norvegicus P17425 rat, cytosolic isoform
-
Rattus norvegicus P22791 rat, mitochondrial isoform
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae P54839 baker's yeast
-
Schizosaccharomyces pombe P54874
-
-
Sus scrofa O02734 pig, mitochondrial isoform
-

Purification (Commentary)

Purification (Comment) Organism
several mutants partially purified Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Methanothermobacter thermautotrophicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mesocricetus auratus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Arabidopsis thaliana (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Borreliella burgdorferi (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Caenorhabditis elegans (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Cricetulus griseus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Pinus sylvestris (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Schizosaccharomyces pombe (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + H2O
-
 Gallus gallus acetate + CoA
-
 ?