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Literature summary for 2.3.3.10 extracted from
- Catalytic properties of recombinant 3-hydroxy-3-methylglutaryl coenzyme A synthase-1 from Blattella germanica (1997), Insect Biochem. Mol. Biol., 27, 499-505.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 cytosolic HMG-CoA synthase genes, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S | Blattella germanica |
| intronless gene, encodes for an active enzyme able to complement eukaryotic Mev-1 cells | Blattella germanica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | - |
Blattella germanica |  |
| acetoacetyl-CoA | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
acetoacetyl-CoA | - |
Saccharomyces cerevisiae | |
| 0.014 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
| 0.0152 | - |
acetyl-CoA | - |
Blattella germanica | |
| 0.051 | - |
acetyl-CoA | - |
Bos taurus | |
| 0.2 | - |
acetyl-CoA | - |
Diploptera punctata | |
| 0.3 | - |
acetyl-CoA | - |
Gallus gallus | |
| 0.4 | - |
acetyl-CoA | - |
Locusta migratoria | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Gallus gallus | 5829 | - |
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| cytosol | - |
Blattella germanica | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE, immunoblot by antibodies, prediction from cDNA nucleotide sequence | Blattella germanica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Bos taurus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Locusta migratoria | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Diploptera punctata | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Blattella germanica | regulation of isoprenoid synthesis and metabolism | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blattella germanica | - |
cockroach | - |
| Bos taurus | - |
ox | - |
| Diploptera punctata | - |
- |
- |
| Gallus gallus | - |
chicken | - |
| Locusta migratoria | - |
- |
- |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Blattella germanica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corpus allatum | - |
Locusta migratoria | - |
| corpus allatum | - |
Diploptera punctata | - |
| fat body | - |
Blattella germanica | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Bos taurus | - |
| ovary | - |
Blattella germanica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Blattella germanica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Bos taurus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Locusta migratoria | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Diploptera punctata | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | regulation of isoprenoid synthesis and metabolism | Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| additional information | - |
Gallus gallus | ? | - |
? | |
| additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | first organism known to have 2 functional cytosolic HMG-CoA synthases | Blattella germanica | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00126 | - |
acetoacetyl-CoA | - |
Blattella germanica | |
| 0.0035 | - |
acetoacetyl-CoA | - |
Bos taurus | |
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