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Literature summary for 2.3.3.10 extracted from

  • Reed, W.D.; Clinkenbeard, K.D.; Lane, M.D.
    Molecular and catalytic properties of mitochondrial (ketogenic) 3-hydroxy-3-methylglutaryl coenzyme A synthase of liver (1975), J. Biol. Chem., 250, 3117-3123.
    View publication on PubMed
Search for more literature for 2.3.3.10

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
 Gallus gallus
acetoacetyl-CoA
-
 Rattus norvegicus
Mg2+ mitochondrial enzyme Gallus gallus
palmitoyl-CoA mitochondrial synthase Gallus gallus
succinyl-CoA mitochondrial synthase, 50% inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 acetoacetyl-CoA
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-
cytoplasm distinct mitochondrial and cytoplasmic forms Gallus gallus 5737
-
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Rattus norvegicus 5829
-
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion distinct mitochondrial and cytoplasmic forms Gallus gallus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cytoplasmic enzyme Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Gallus gallus
57000
-
 2 * 57000, gel filtration Gallus gallus
96000
-
 gel filtration Gallus gallus
105000
-
 sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 chicken
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial form Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, purified synthase may be stored for 2-3 months with little loss of activity by adding 30% glyerol Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Gallus gallus
dimer 2 * 57000, gel filtration Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0005
-
 palmityl-CoA
-
 Gallus gallus