Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Asp + ergosterol | Aspergillus oryzae | - |
AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + ergosterol | Aspergillus oryzae ATCC 42149 | - |
AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + tRNAAsn | Aspergillus oryzae | - |
AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + Asp + tRNAAsn | Aspergillus oryzae ATCC 42149 | - |
AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | Aspergillus oryzae | - |
ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | Aspergillus oryzae ATCC 42149 | - |
ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
additional information | Aspergillus oryzae | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | ? | - |
- |
|
additional information | Aspergillus oryzae ATCC 42149 | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WKG3 | cf. EC 6.1.1.12 | - |
Aspergillus fumigatus ATCC MYA-4609 | Q4WKG3 | cf. EC 6.1.1.12 | - |
Aspergillus oryzae | Q2URG4 | cf. EC 6.1.1.12 | - |
Aspergillus oryzae ATCC 42149 | Q2URG4 | cf. EC 6.1.1.12 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus oryzae | - |
mycelium | - |
Aspergillus fumigatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Asp + ergosterol | - |
Aspergillus oryzae | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + ergosterol | - |
Aspergillus fumigatus | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + ergosterol | - |
Aspergillus fumigatus ATCC MYA-4609 | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + ergosterol | - |
Aspergillus oryzae ATCC 42149 | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + Asp + tRNAAsn | - |
Aspergillus oryzae | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + Asp + tRNAAsn | - |
Aspergillus fumigatus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + Asp + tRNAAsn | - |
Aspergillus fumigatus ATCC MYA-4609 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + Asp + tRNAAsn | - |
Aspergillus oryzae ATCC 42149 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + ergosterol | - |
Aspergillus oryzae | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ATP + L-aspartate + ergosterol | - |
Aspergillus oryzae ATCC 42149 | AMP + diphosphate + ergosteryl-3beta-O-L-aspartate | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | - |
Aspergillus oryzae | ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | - |
Aspergillus fumigatus | ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | - |
Aspergillus fumigatus ATCC MYA-4609 | ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
ergosterol + L-aspartyl-tRNAAsn | - |
Aspergillus oryzae ATCC 42149 | ergosteryl-3beta-O-L-aspartate + tRNAAsn | - |
? | |
additional information | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | Aspergillus oryzae | ? | - |
- |
|
additional information | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | Aspergillus fumigatus | ? | - |
- |
|
additional information | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
- |
|
additional information | a bifunctional enzyme, ergosteryl-3beta-O-L-aspartate synthase ErdS, is responsible for synthesis of ergosteryl-3beta-O-L-aspartate. ErdS produces aspartyl-tRNAAsp, reaction of EC 6.1.1.12, and transfers aspartate from Asp-tRNAAsp onto ergosterol | Aspergillus oryzae ATCC 42149 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AFUA_1g02570 | - |
Aspergillus fumigatus |
AO090005000838 | - |
Aspergillus oryzae |
DUF2156 domain protein | - |
Aspergillus oryzae |
DUF2156 domain protein | - |
Aspergillus fumigatus |
ErdS | - |
Aspergillus oryzae |
ErdS | - |
Aspergillus fumigatus |
ergosteryl-3beta-O-L-aspartate synthase | - |
Aspergillus oryzae |
ergosteryl-3beta-O-L-aspartate synthase | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
evolution | ErdS corresponds to a unique fusion of an aspartyl-tRNA synthetase that produces aspartyl-tRNAAsp and of a Domain of Unknown Function 2156, which actually transfers aspartate from Asp-tRNAAsp onto ergosterol. The entire ergosteryl-3beta-O-L-aspartate synthesis/degradation pathway is conserved across higher fungi | Aspergillus oryzae |
evolution | ErdS corresponds to a unique fusion of an aspartyl-tRNA synthetase that produces aspartyl-tRNAAsp and of a Domain of Unknown Function 2156, which actually transfers aspartate from Asp-tRNAAsp onto ergosterol. The entire ergosteryl-3beta-O-L-aspartate synthesis/degradation pathway is conserved across higher fungi | Aspergillus fumigatus |
physiological function | ergosteryl-3beta-O-L-aspartate is a type of sterol conjugate, specific to fungi, that is produced by ErdS enzymes, through a tRNA-dependent process. Fusion of the AspRS and the transferase (DUF2156) domain is required for full activity. ErdS mutants grow normally on solid media. Removal of the Asp group from ergosteryl-3beta-O-L-aspartate is catalyzed by a second enzyme, ErdH, that is a ergosteryl-3beta-O-L-aspartate hydrolase participating in the turnover of the conjugated sterol in vivo | Aspergillus fumigatus |
physiological function | ergosteryl-3beta-O-L-aspartate is a type of sterol conjugate, specific to fungi, that is produced by ErdSs enzymes, through a tRNA-dependent process. Fusion of the AspRS and the transferase (DUF2156) domain is required for full activity. ErdS mutants grow normally on solid media. Removal of the Asp group from ergosteryl-3beta-O-L-aspartate is catalyzed by a second enzyme, ErdH, that is a ergosteryl-3beta-O-L-aspartate hydrolase participating in the turnover of the conjugated sterol in vivo | Aspergillus oryzae |