Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
N-terminal domain in complex with von Hippel-Lindau tumor suppressor protein, elonhin B, and elongin C, hanging drop vapor diffusion method, using 5 M sodium formate as precipitant | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine | Homo sapiens | - |
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P40337 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, glutatione S-transferase column chromatography, maltose binding protein-Trap column chromatography, anion exchange chromatorgaphy, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine | - |
Homo sapiens | [cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CRL | - |
Homo sapiens |
Cul2 E3 ligase | - |
Homo sapiens |
cullin 2 E3 ubiquitin ligase | - |
Homo sapiens |
cullin-RING E3 ubiquitin ligase | - |
Homo sapiens |