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Literature summary for 2.3.2.7 extracted from
- Novel properties of gamma-glutamyltransferase from Pseudomonas syringae with beta-aspartyltransferase activity (2015), Biotechnol. Lett., 37, 2255-2263.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | slight inhibition at 5 mM | Pseudomonas syringae |  |
| EDTA | slight inhibition at 5 mM | Pseudomonas syringae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + hydroxylamine | Pseudomonas syringae | - |
NH3 + beta-L-aspartylhydroxamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae | - |
pv. phaseolicola 1448A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Cellufine column chromatography, butyl Toyopearl column chromatography, and Hitrap CM column chromatography | Pseudomonas syringae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + hydroxylamine | - |
Pseudomonas syringae | NH3 + beta-L-aspartylhydroxamate | - |
? | |
| L-asparagine + hydroxylamine | 301% activity compared to gamma-glutamyl-4-nitroanilide, hydroxylamine is the only substrate acceptor | Pseudomonas syringae | NH3 + beta-L-aspartylhydroxamate | - |
? | |
| additional information | no activity with glycylglycine, ethylamine, and methylamine | Pseudomonas syringae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAT | - |
Pseudomonas syringae |
| BAT-GGT | bifunctional enzyme | Pseudomonas syringae |
| beta-aspartyl-gamma-glutamyltransferase | bifunctional enzyme | Pseudomonas syringae |
| beta-aspartyltransferase | - |
Pseudomonas syringae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas syringae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | more than 40% activity between 30 and 60°C | Pseudomonas syringae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | after 10 min incubation at 30, 50, 50, 60 and 70°C, the enzyme shows 100, 95, 90, 85, and 60% residual activity, respectively | Pseudomonas syringae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas syringae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | more than 40% activity between pH 6.0 and 11.0 | Pseudomonas syringae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | after 10 min incubation at pH 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0, the enzyme shows 30, 20, 55, 100, 95, 80, and 60% residual activity, respectively | Pseudomonas syringae |
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Release 2023.1 (January 2023)
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