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Literature summary for 2.3.2.6 extracted from

  • Ebhardt, H.; Xu, Z.; Fung, A.; Fahlman, R.
    Quantification of the post-translational addition of amino acids to proteins by MALDI-TOF mass spectrometry (2009), Anal. Chem., 81, 1937-1943.
    View publication on PubMed
Search for more literature for 2.3.2.6

Application

Application Comment Organism
analysis a novel method to quantify L/F transferase activity by matrix assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF, is reported Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pCA24N Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-azido phenylalanine, Km apparent Escherichia coli
3.2
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, phenylalanine, Km apparent Escherichia coli
4.3
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Km apparent Escherichia coli
18
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-acetyl-N-(tert-butoxycarbonyl)-L-phenylalanine, Km apparent Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucyl-tRNALeu + protein Escherichia coli
-
 tRNALeu + L-leucyl-protein
-
 ?
L-phenylalanyl-tRNA + protein Escherichia coli
-
 tRNA + L-phenylalanyl-protein
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucyl-tRNA + REPGLCTWQSLR substrate peptide Escherichia coli tRNA + LREPGLCTWQSLR
-
 ?
L-leucyl-tRNALeu + protein
-
 Escherichia coli tRNALeu + L-leucyl-protein
-
 ?
L-phenylalanyl-tRNA + protein
-
 Escherichia coli tRNA + L-phenylalanyl-protein
-
 ?
L-phenylalanyl-tRNA + REPGLCTWQSLR substrate peptide Escherichia coli tRNA + FREPGLCTWQSLR
-
 ?

Synonyms

Synonyms Comment Organism
L/F transferase
-
 Escherichia coli
leucyl/phenylalanyl-tRNA-protein transferase
-
 Escherichia coli
leucyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 peptide bond formation assay Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00183
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-azido phenylalanine, Kcat apparent Escherichia coli
0.00283
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-acetyl-N-(tert-butoxycarbonyl)-L-phenylalanine, Kcat apparent Escherichia coli
0.00333
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Kcat apparent Escherichia coli
0.00367
-
 REPGLCTWQSLR amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, phenylalanine, Kcat apparent Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 peptide bond formation assay Escherichia coli