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Literature summary for 2.3.2.5 extracted from

  • Carrillo, D.R.; Parthier, C.; Jaenckel, N.; Grandke, J.; Stelter, M.; Schilling, S.; Boehme, M.; Neumann, P.; Wolf, R.; Demuth, H.U.; Stubbs, M.T.; Rahfeld, J.U.
    Kinetic and structural characterization of bacterial glutaminyl cyclases from Zymomonas mobilis and Myxococcus xanthus (2010), Biol. Chem., 391, 1419-1428.
    View publication on PubMed
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zymomonas mobilis
expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M MES and 30% (w/v) PEG-4000 Myxococcus xanthus
hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate and 12% (w/v) PEG-4000 Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
E48Q the mutant’s pH optimum is shifted towards higher pH values Zymomonas mobilis
Q46E the mutant’s pH optimum is shifted towards lower pH values, although the activity towards L-glutamine-2-naphtylamine is some 1000fold lower than the wild type Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.1
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.1
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.1
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.2
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.3
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.4
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.4
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.5
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.7
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.8
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
0.9
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
1
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
1
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
1.1
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
1.1
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
1.6
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
1.9
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.1
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.4
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.5
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.7
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.9
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.9
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
3.5
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
3.8
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
4.2
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
5.6
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
6.5
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
8.2
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
20.4
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
24.4
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29900
-
 x * 29900, MALDI-TOF mass spectrometry Zymomonas mobilis
30000
-
 x * 30000, SDS-PAGE Zymomonas mobilis
30000
-
 x * 30000, SDS-PAGE Myxococcus xanthus
31100
-
 x * 31100, MALDI-TOF mass spectrometry Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q1DDS6
-
-
Zymomonas mobilis Q5NLA9
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Zymomonas mobilis
Q-Sepharose column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln-2-naphthylamide
-
 Zymomonas mobilis 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
L-Gln-2-naphthylamide
-
 Myxococcus xanthus 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
L-Gln-7-amido-4-methylcoumarin
-
 Zymomonas mobilis 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
L-Gln-7-amido-4-methylcoumarin
-
 Myxococcus xanthus 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
L-Gln-amide
-
 Zymomonas mobilis 5-oxoprolyl-amide + NH3
-
 ?
L-Gln-amide
-
 Myxococcus xanthus 5-oxoprolyl-amide + NH3
-
 ?
L-Gln-Gly
-
 Zymomonas mobilis 5-oxoprolylglycine + NH3
-
 ?
L-Gln-L-Gln
-
 Zymomonas mobilis 5-oxoprolyl-L-Gln + NH3
-
 ?
L-Gln-L-Gln
-
 Myxococcus xanthus 5-oxoprolyl-L-Gln + NH3
-
 ?
L-Gln-L-Glu
-
 Zymomonas mobilis 5-oxoprolyl-L-Glu + NH3
-
 ?
L-Gln-L-Glu
-
 Myxococcus xanthus 5-oxoprolyl-L-Glu + NH3
-
 ?
L-Gln-tert-butyl ester
-
 Zymomonas mobilis 5-oxoprolyl-tert-butyl ester + NH3
-
 ?
L-Gln-tert-butyl ester
-
 Myxococcus xanthus 5-oxoprolyl-tert-butyl ester + NH3
-
 ?
additional information the enzyme shows no activity towards L-Gln-Gly Myxococcus xanthus ?
-
 ?
additional information the enzyme shows no activity towards QAAR Zymomonas mobilis ?
-
 ?
QAAE
-
 Zymomonas mobilis 5-oxoprolyl-AAE + NH3
-
 ?
QAAE
-
 Myxococcus xanthus 5-oxoprolyl-AAE + NH3
-
 ?
QAAF
-
 Zymomonas mobilis 5-oxoprolyl-AAF + NH3
-
 ?
QAAF
-
 Myxococcus xanthus 5-oxoprolyl-AAF + NH3
-
 ?
QAAR
-
 Myxococcus xanthus 5-oxoprolyl-AAR + NH3
-
 ?
QAEA
-
 Zymomonas mobilis 5-oxoprolyl-AEA + NH3
-
 ?
QAEA
-
 Myxococcus xanthus 5-oxoprolyl-AEA + NH3
-
 ?
QAFA
-
 Zymomonas mobilis 5-oxoprolyl-AFA + NH3
-
 ?
QAFA
-
 Myxococcus xanthus 5-oxoprolyl-AFA + NH3
-
 ?
QARA
-
 Zymomonas mobilis 5-oxoprolyl-ARA + NH3
-
 ?
QARA
-
 Myxococcus xanthus 5-oxoprolyl-ARA + NH3
-
 ?
QEAA
-
 Zymomonas mobilis 5-oxoprolyl-EAA + NH3
-
 ?
QEAA
-
 Myxococcus xanthus 5-oxoprolyl-EAA + NH3
-
 ?
QFAA
-
 Zymomonas mobilis 5-oxoprolyl-FAA + NH3
-
 ?
QFAA
-
 Myxococcus xanthus 5-oxoprolyl-FAA + NH3
-
 ?
QGGG
-
 Zymomonas mobilis 5-oxoprolyl-GGG + NH3
-
 ?
QGGG
-
 Myxococcus xanthus 5-oxoprolyl-GGG + NH3
-
 ?
QRAA
-
 Zymomonas mobilis 5-oxoprolyl-RAA + NH3
-
 ?
QRAA
-
 Myxococcus xanthus 5-oxoprolyl-RAA + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Zymomonas mobilis
? x * 30000, SDS-PAGE Myxococcus xanthus
? x * 29900, MALDI-TOF mass spectrometry Zymomonas mobilis
? x * 31100, MALDI-TOF mass spectrometry Myxococcus xanthus

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
 Zymomonas mobilis
glutaminyl cyclase
-
 Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00008
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0032
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0042
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0064
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.007
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0074
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.012
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.021
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.054
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.084
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.27
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.45
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
1.1
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.7
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
7.1
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
12.8
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
17.9
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
18.9
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
31.7
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
32
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
35.7
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
40
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
40
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
60
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
61.5
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
64.9
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
66
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
102
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
128
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
129
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
170
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
183
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Zymomonas mobilis
8
-
-
 Myxococcus xanthus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0008
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0013
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0021
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0026
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0031
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0049
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.0067
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.013
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.02
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.034
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
0.24
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
1.46
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
4.8
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
5.4
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
6.64
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
8.5
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
9
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
13
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
15.7
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
18
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
21.2
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
31
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
35
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
36
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
41
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
55
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
63.6
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
76
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
142
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
219
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
412
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
1215
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus