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Literature summary for 2.3.2.5 extracted from

  • Ruiz-Carrillo, D.; Koch, B.; Parthier, C.; Wermann, M.; Dambe, T.; Buchholz, M.; Ludwig, H.H.; Heiser, U.; Rahfeld, J.U.; Stubbs, M.T.; Schilling, S.; Demuth, H.U.
    Structures of glycosylated mammalian glutaminyl cyclases reveal conformational variability near the active center (2011), Biochemistry, 50, 6280-6288.
    View publication on PubMed
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM imidazole (pH 8.0), 30% (v/v) 2-methyl-2,4-pentanediol, and 11% (w/v) 4000 PEG Homo sapiens
hanging drop vapor diffusion method, using 100 mM sodium acetate (pH 5.3), 200 mM ammonium sulfate, and 12% (w/v) 2000 MME-PEG Mus musculus

Protein Variants

Protein Variants Comment Organism
W207F the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected Homo sapiens
W207L the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected Homo sapiens
W207Q the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-Methylimidazole
-
 Homo sapiens
1-Methylimidazole
-
 Mus musculus
benzimidazole
-
 Homo sapiens
benzimidazole
-
 Mus musculus
benzylimidazole
-
 Homo sapiens
benzylimidazole
-
 Mus musculus
cysteamine
-
 Homo sapiens
cysteamine
-
 Mus musculus
imidazole
-
 Homo sapiens
imidazole
-
 Mus musculus
Nomega-acetylhistamine
-
 Homo sapiens
Nomega-acetylhistamine
-
 Mus musculus
PBD150 highly potent inhibitor Homo sapiens
PBD150 highly potent inhibitor Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme contains a catalytic zinc ion Homo sapiens
Zn2+ the enzyme contains a catalytic zinc ion Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16769
-
-
Mus musculus Q9CYK2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
glycoprotein
-
 Mus musculus

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus
chelating Sepharose column chromatography and butyl Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln-2-naphthylamide
-
 Homo sapiens 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
L-Gln-2-naphthylamide
-
 Mus musculus 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
L-Gln-7-amido-4-methylcoumarin
-
 Homo sapiens 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
L-Gln-7-amido-4-methylcoumarin
-
 Mus musculus 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
L-Gln-Gly-L-Pro
-
 Homo sapiens 5-oxoprolyl-Gly-L-Pro + NH3
-
 ?
L-Gln-Gly-L-Pro
-
 Mus musculus 5-oxoprolyl-Gly-L-Pro + NH3
-
 ?
L-Gln-L-Gln
-
 Homo sapiens 5-oxoprolyl-L-Gln + NH3
-
 ?
L-Gln-L-Gln-OH
-
 Mus musculus 5-oxoprolyl-L-Gln + NH3
-
 ?
L-Gln-L-Glu
-
 Homo sapiens 5-oxoprolyl-L-Glu + NH3
-
 ?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
-
 Homo sapiens 5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
-
 ?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
-
 Mus musculus 5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
-
 ?
L-Gln-L-Glu-OH
-
 Mus musculus 5-oxoprolyl-L-Glu + NH3
-
 ?
L-Gln-L-Phe-L-Ala-NH2
-
 Homo sapiens 5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
-
 ?
L-Gln-L-Phe-L-Ala-NH2
-
 Mus musculus 5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
 Homo sapiens
glutaminyl cyclase
-
 Mus musculus