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Literature summary for 2.3.2.5 extracted from

  • Huang, K.F.; Wang, Y.R.; Chang, E.C.; Chou, T.L.; Wang, A.H.
    A conserved hydrogen-bond network in the catalytic centre of animal glutaminyl cyclases is critical for catalysis (2008), Biochem. J., 411, 181-190.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
D248A kcat/KM for Gln-2-naphthylamide is 136fold lower than wild-type value Homo sapiens
D248Q kcat/KM for Gln-2-naphthylamide is 7123fold lower than wild-type value Homo sapiens
D305A kcat/KM for Gln-2-naphthylamide is 1493fold lower than wild-type value Homo sapiens
D305E kcat/KM for Gln-2-naphthylamide is 21000fold lower than wild-type value Homo sapiens
D305N kcat/KM for Gln-2-naphthylamide is 772fold lower than wild-type value Homo sapiens
E201D kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value Homo sapiens
H319L kcat/KM for Gln-2-naphthylamide is 15fold lower than wild-type value Homo sapiens
S160A kcat/KM for Gln-2-naphthylamide is 3fold lower than wild-type value Homo sapiens
S160G kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
Gln-2-naphthylamide pH 8.0, 25°C, wild-type enzyme Homo sapiens
0.125
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D248A Homo sapiens
0.161
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme H319L Homo sapiens
0.174
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme S160A Homo sapiens
0.254
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305N Homo sapiens
0.33
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D248Q Homo sapiens
0.431
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305E Homo sapiens
0.615
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme S160G Homo sapiens
0.736
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme E201D Homo sapiens
1.1
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hanging-drop vapour-diffusion method, wild-type and mutant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln-2-naphthylamide
-
Homo sapiens 2-oxoprolyl-2-naphthylamide + NH3
-
?
Gln-4-methylcoumarinylamide
-
Homo sapiens 2-oxoprolyl-4-methylcoumarinylamide + NH3
-
?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305E Homo sapiens
0.0042
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D248Q Homo sapiens
0.03
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305N Homo sapiens
0.068
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D305A Homo sapiens
0.085
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme D248A Homo sapiens
0.84
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme S160G Homo sapiens
1
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme E201D Homo sapiens
1
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme H319L Homo sapiens
5.3
-
Gln-2-naphthylamide pH 8.0, 25°C, mutant enzyme S160A Homo sapiens
5.4
-
Gln-2-naphthylamide pH 8.0, 25°C, wild-type enzyme Homo sapiens