Literature summary for 2.3.2.5 extracted from

Bateman, R.C., Jr.; Temple, J.S.; Misquitta, S.A.; Booth, R.E.
Evidence for essential histidines in human pituitary glutaminyl cyclase (2001), Biochemistry, 40, 11246-11250.

Search for more literature for 2.3.2.5

Protein Variants

Protein Variants	Comment	Organism
H140Q	inactive enzyme	Homo sapiens
H307Q	mutant enzyme with increased KM-value	Homo sapiens
H319Q	mutant enzyme with increased KM-value	Homo sapiens
H330Q	inactive enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyl dicarbonate	rapid inactivation by modification of three essential His residues, at neutral pH, partial reactivation with hydroxylamine	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	Gln-NH2	wild-type enzyme	Homo sapiens
4.3	-	Gln-NH2	mutant enzyme H307Q	Homo sapiens
4.9	-	Gln-NH2	mutant enzyme H319Q	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pituitary gland	-	Homo sapiens	-

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.36	-	Gln-NH2	mutant enzyme H319Q	Homo sapiens
0.388	-	Gln-NH2	mutant enzyme H307Q	Homo sapiens
0.435	-	Gln-NH2	unmutated enzyme	Homo sapiens

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Release 2023.1 (January 2023)