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Literature summary for 2.3.2.5 extracted from
- Molecular cloning, sequence analysis and expression of human pituitary glutaminyl cyclase (1994), J. Mol. Endocrinol., 13, 77-86.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the Escherichia coli expression vectors pMALc2 and pET19b. Expression of this cDNA in either vector results in the production of a glutaminyl cyclase fusion protein which is enzymatically active and reacts with anti-bovine glutaminyl cyclase antisera | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
Gln-Gln-Gln | - |
Homo sapiens |  |
| 0.64 | - |
Gln-Gln | - |
Homo sapiens | |
| 1.3 | - |
Gln-Ala | - |
Homo sapiens | |
| 1.88 | - |
Gln-Gly | - |
Homo sapiens | |
| 7.15 | - |
Gln-NH2 | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Homo sapiens |
| 40876 | - |
x * 40876, calculation from nucleotide sequence | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pituitary gland | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gln-Ala | - |
Homo sapiens | Glp-Ala + NH3 | - |
? | |
| Gln-Gln | - |
Homo sapiens | Glp-Gln + NH3 | - |
? | |
| Gln-Gln-Gln | - |
Homo sapiens | Glp-Gln-Gln + NH3 | - |
? | |
| Gln-Gly | - |
Homo sapiens | Glp-Gly + NH3 | - |
? | |
| Gln-NH2 | - |
Homo sapiens | Glp-amide + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Homo sapiens |
| ? | x * 40876, calculation from nucleotide sequence | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.368 | - |
Gln-Gly | - |
Homo sapiens | |
| 0.55 | - |
Gln-NH2 | - |
Homo sapiens | |
| 0.755 | - |
Gln-Gln-Gln | - |
Homo sapiens | |
| 0.848 | - |
Gln-Gln | - |
Homo sapiens | |
| 1.27 | - |
Gln-Ala | - |
Homo sapiens | |
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