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Literature summary for 2.3.2.31 extracted from

  • Kelsall, I.; Duda, D.; Olszewski, J.; Hofmann, K.; Knebel, A.; Langevin, F.; Wood, N.; Wightman, M.; Schulman, B.; Alpi, A.
    TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes (2013), EMBO J., 32, 2848-2860 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.31

Activating Compound

Activating Compound Comment Organism Structure
additional information neddylated cullin-1-RBX1 activates auto-inhibited isoform HHARI Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[E2 ubiquitin-conjugating enzyme cullin-1]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-1]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-2]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-2]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-4A]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for HHARI [E2 ubiquitin-conjugating enzyme cullin-4A]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-5]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for TRIAD1 [E2 ubiquitin-conjugating enzyme cullin-5]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme UBCH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine Homo sapiens overall reaction for TRIAD1 [E2 ubiquitin-conjugating enzyme UBCH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GSH-Sepharose column chromatography, amylose resin column chromatography, and Ni2+-Sepharose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme-substrate-interaction is neddylation-dependent Homo sapiens ?
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-1]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-1]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-2]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-2]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-4A]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for HHARI Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-4A]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme cullin-5]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for TRIAD1 Homo sapiens [E2 ubiquitin-conjugating enzyme cullin-5]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?
[E2 ubiquitin-conjugating enzyme UBCH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine overall reaction for TRIAD1 Homo sapiens [E2 ubiquitin-conjugating enzyme UBCH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
HHARI
-
 Homo sapiens
RBR-type E3 ligase
-
 Homo sapiens
Triad1
-
 Homo sapiens