Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.27 extracted from

  • Hodge, C.D.; Ismail, I.H.; Edwards, R.A.; Hura, G.L.; Xiao, A.T.; Tainer, J.A.; Hendzel, M.J.; Glover, J.N.
    RNF8 E3 ubiquitin ligase stimulates Ubc13 E2 conjugating activity that is essential for DNA double-strand break signaling and BRCA1 tumor suppressor recruitment (2016), J. Biol. Chem., 291, 9396-9410.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.27

Crystallization (Commentary)

Crystallization (Comment) Organism
RNF8(345-485)/Ubc13-Ub complex, hanging drop vapor diffusion method, using Homo sapiens

Protein Variants

Protein Variants Comment Organism
L451D the mutation causes a drastic decrease in the ability of the enzyme to stimulate Ubc13 Homo sapiens
R441A the mutation has little effect on the enzyme activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + [acceptor protein]-L-lysine Homo sapiens
-
 [E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O76064
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + [acceptor protein]-L-lysine
-
 Homo sapiens [E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
RING E3 ubiquitin ligase
-
 Homo sapiens
RNF8
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme activity is essential for DNA double-strand break signaling and necessary for downstream recruitment of 53BP1 and BRCA1 proteins Homo sapiens