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Literature summary for 2.3.2.27 extracted from

  • Gao, W.; Liu, W.; Zhao, M.; Li, W.X.
    NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis (2015), Nucleic Acids Res., 43, 607-617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C367S mutation within RING finger domain, mutant protein does not display significant ubiquitin ligase activity Arabidopsis thaliana
H348Y mutation within RING finger domain, mutant protein does not display significant ubiquitin ligase activity Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SYH3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
additional information expression in all developmental stages Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[UbcH5b]-L-cysteine + [NERF]-L-lysine
-
 Arabidopsis thaliana [UbcH5b]-L-cysteine + N6-ubiquitinyl-[NERF]-L-lysine autoubiquitylation reaction ?

Synonyms

Synonyms Comment Organism
At1g54150
-
 Arabidopsis thaliana
NERF
-
 Arabidopsis thaliana
NFYA5 enhancing RING FINGER
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression is induced by drought stress up

General Information

General Information Comment Organism
physiological function isoform NERF is the cis-natural antisense transcript gene of NFYA5. NERF can produce siRNAs from their overlapping region and affect NFYA5 transcripts by functioning together with miR169. The NERF protein functions as an E3 ligase for ubiquitination. Overexpression of NERF or overlapping region cDNA leads to siRNA-NERF accumulation, miR169 repression, and NFYA5 transcript enhancement. Knockdown of NERF transcripts by an artificial miRNA enhances miR169 abundance and reduces NFYA5 transcripts Arabidopsis thaliana