Application | Comment | Organism |
---|---|---|
synthesis | the recombinant extracellular enzyme is used for enzymatic synthesis of L-theanine, which is performed at optimal conditions: 20 mM L-Gln, 100 mM ethylamine-HCl, 0.5 U/ml BaGGT, incubated at 40°C for 6 h, 200 rpm | Bacillus amyloliquefaciens |
Cloned (Comment) | Organism |
---|---|
gene ggt, recombinant expression of His6-tagged enzyme in Bacillus subtilis strain 168 (pHT43-spAmyQ-ggtDELTAsp) under control of the Plac promoter, extracellular secretion, subcloning in Escherichia coli strain DH5alpha, method optimization. Dual-signal peptides promote more secretion than a single signal peptide | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.214 | - |
L-gamma-glutamyl-4-nitroanilide | recombinant extracellular enzyme, pH 9.0, 40°C | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | Bacillus amyloliquefaciens | - |
a peptide + a 5-L-glutamyl amino acid | - |
? | |
a (5-L-glutamyl)-peptide + an amino acid | Bacillus amyloliquefaciens BH072 | - |
a peptide + a 5-L-glutamyl amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens BH072 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged eextracellular enzyme from Bacillus subtilis strain 168 by nickel affinity chromatography | Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55 | - |
purified recombinant extracellular enzyme, pH 9.0, 40°C, substrate L-gamma-glutamyl-4-nitroanilide | Bacillus amyloliquefaciens |
Storage Stability | Organism |
---|---|
40°C, purified recombinant extracellular enzyme, 72 h, 90% of initial activity remaining, and more than 50% of initial activity after being maintained at different pH values for 24 h | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | - |
Bacillus amyloliquefaciens | a peptide + a 5-L-glutamyl amino acid | - |
? | |
a (5-L-glutamyl)-peptide + an amino acid | - |
Bacillus amyloliquefaciens BH072 | a peptide + a 5-L-glutamyl amino acid | - |
? | |
L-gamma-glutamyl-4-nitroanilide + glycylglycine | - |
Bacillus amyloliquefaciens | 4-nitroaniline + 5-L-glutamyl-glycylglycine | - |
? | |
L-gamma-glutamyl-4-nitroanilide + glycylglycine | - |
Bacillus amyloliquefaciens BH072 | 4-nitroaniline + 5-L-glutamyl-glycylglycine | - |
? | |
L-glutamine + ethylamine | GGT transfers the gamma-glutamyl moiety from L-Gln to ethylamine to form the product gamma-glutamylethylamine (L-theanine), product identification by mass spectrometry, optimization of reaction conditions, overview | Bacillus amyloliquefaciens | NH3 + gamma-glutamylethylamine | - |
? | |
L-glutamine + ethylamine | GGT transfers the gamma-glutamyl moiety from L-Gln to ethylamine to form the product gamma-glutamylethylamine (L-theanine), product identification by mass spectrometry, optimization of reaction conditions, overview | Bacillus amyloliquefaciens BH072 | NH3 + gamma-glutamylethylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 46000, large subunit, + 1 * 22000, small subunit, His6-tagged enzyme, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
BaGGT | - |
Bacillus amyloliquefaciens |
gamma-glutamyltranspeptidase | - |
Bacillus amyloliquefaciens |
GGT | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 40/56/34/20/6% relative activity at 20/30/50/60/70°C, respectively, recombinant enzyme, profile overview | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | there is hardly any activity loss of the purified recombinant enzyme after preincubation at 40 °C for 72 h, but the activity drops to 7.5% after preincubation for 8 h at 50°C | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 46/75/64% relative activity at pH 7.0/8.0/10.0, respectively, recombinant enzyme, profile overview | Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 25°C, 7 h, 80% activity or higher is retained at pH 7.0-10.0 and, in all cases, it is above 50% at pH 5.0-11.0 | Bacillus amyloliquefaciens |