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Literature summary for 2.3.2.2 extracted from

  • Spitzmüller, Z.; Gonda, S.; Kiss-Szikszai, A.; Vasas, G.; Pocsi, I.; Emri, T.
    Characterization of extracellular gamma-glutamyl transpeptidase from Aspergillus nidulans (2016), Mycoscience, 57, 400-403 .
No PubMed abstract available
Search for more literature for 2.3.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics with L-gamma-glutamyl-4-nitroanilide as substrate, native extracellular enzyme Aspergillus nidulans
0.2
-
 L-gamma-glutamyl-4-nitroanilide pH 8.0, 37°C, native extracellular enzyme Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus nidulans
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (5-L-glutamyl)-peptide + an amino acid Aspergillus nidulans
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Aspergillus nidulans FGSC A26
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme partially from carbon stressed cultures Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture carbon stressed cultures Aspergillus nidulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (5-L-glutamyl)-peptide + an amino acid
-
 Aspergillus nidulans a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Aspergillus nidulans FGSC A26 a peptide + a 5-L-glutamyl amino acid
-
 ?
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine GSH Aspergillus nidulans L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
-
 ?
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine GSH Aspergillus nidulans FGSC A26 L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
-
 ?
glutathione disulfide + glycylglycine GSSG Aspergillus nidulans L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
glutathione disulfide + glycylglycine GSSG Aspergillus nidulans FGSC A26 L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Aspergillus nidulans 4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Aspergillus nidulans FGSC A26 4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
 ?
L-glutamine + glycylglycine
-
 Aspergillus nidulans NH3 + 5-L-glutamyl-glycylglycine
-
 ?
additional information the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors Aspergillus nidulans ?
-
-
additional information the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors Aspergillus nidulans FGSC A26 ?
-
-

Synonyms

Synonyms Comment Organism
AngammaGT
-
 Aspergillus nidulans
gamma-glutamyl transpeptidase
-
 Aspergillus nidulans
gammaGT
-
 Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
7 10 no significant activities are detected below pH 7.0 or above pH 10.0 Aspergillus nidulans

General Information

General Information Comment Organism
malfunction deletion of the ggtA (AN10444) encoding a putative gammaGT decreased the gammaGT activities below to the detection limit suggesting that GgtA is the only enzyme possessing significant gammaGT activity under carbon stressed conditions in Aspergillus nidulans Aspergillus nidulans
additional information enzyme AngammaGT has little hydrolase activity (cf. EC 3.4.19.13) Aspergillus nidulans
physiological function the function of this enzyme is not to degrade, but to produce, gamma-glutamyl compounds, which may be related to the utilization of extracellular peptides and amino-acids in carbon stressed cultures Aspergillus nidulans