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Literature summary for 2.3.2.2 extracted from

  • Wada, K.; Irie, M.; Suzuki, H.; Fukuyama, K.
    Crystal structure of the halotolerant gamma-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket (2010), FEBS J., 277, 1000-1009.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-glutamate, hanging drop vapor diffusion method, using poly(ethylene glycol) 4000, 100 mM MES buffer (pH 7.0), 600 mM NaCl, and 5% (v/v) Jeffamine M-600 Bacillus subtilis

General Stability

General Stability Organism
the enzyme retains 86% of its activity even in 3 M NaCl Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
1 * 40000 + 1 * 20000, SDS-PAGE Bacillus subtilis
40000
-
1 * 40000 + 1 * 20000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P54422
-
-
Bacillus subtilis 168 P54422
-
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal His-tagged enzyme is purified by ammonium sulfate precipitation and Sephacryl S-200 gel filtration Bacillus subtilis

Subunits

Subunits Comment Organism
heterodimer 1 * 40000 + 1 * 20000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
gamma-glutamyltranspeptidase
-
Bacillus subtilis
GGT
-
Bacillus subtilis