Literature summary for 2.3.2.2 extracted from

Chang, H.P.; Liang, W.C.; Lyu, R.C.; Chi, M.C.; Wang, T.F.; Su, K.L.; Hung, H.C.; Lin, L.L.
Effects of C-terminal truncation on autocatalytic processing of Bacillus licheniformis gamma-glutamyl transpeptidase (2010), Biochemistry, 75, 919-929.

Search for more literature for 2.3.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli M15 cells	Bacillus licheniformis

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of up to nine amino acid residues from the C-terminal end of the enzyme definitely affects its autocatalytic activity	Bacillus licheniformis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	5-L-glutamyl-4-nitroanilide	in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C	Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65230	-	analytical ultracentrifugation	Bacillus licheniformis

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	Q65KZ6	-	-
Bacillus licheniformis ATCC 14580	Q65KZ6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA resin column chromatography	Bacillus licheniformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-L-glutamyl-2-naphthylamide + glycylglycine	-	Bacillus licheniformis	5-L-glutamyl-glycylglycine + 2-naphthylamine	-	?
5-L-glutamyl-2-naphthylamide + glycylglycine	-	Bacillus licheniformis ATCC 14580	5-L-glutamyl-glycylglycine + 2-naphthylamine	-	?
5-L-glutamyl-4-nitroanilide + glycylglycine	-	Bacillus licheniformis	5-L-glutamyl-glycylglycine + 4-nitroaniline	-	?
5-L-glutamyl-4-nitroanilide + glycylglycine	-	Bacillus licheniformis ATCC 14580	5-L-glutamyl-glycylglycine + 4-nitroaniline	-	?
additional information	the enzyme must undergo autocatalytic processing before it has enzymatic activity	Bacillus licheniformis	?	-	?
additional information	the enzyme must undergo autocatalytic processing before it has enzymatic activity	Bacillus licheniformis ATCC 14580	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	-	Bacillus licheniformis

Synonyms

Synonyms	Comment	Organism
gamma-glutamyl transpeptidase	-	Bacillus licheniformis
GGT	-	Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
97	-	5-L-glutamyl-4-nitroanilide	in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C	Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1245	-	5-L-glutamyl-4-nitroanilide	in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C	Bacillus licheniformis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)