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Literature summary for 2.3.2.2 extracted from

  • Nakano, Y.; Okawa, S.; Yamauchi, T.; Koizumi, Y.; Sekiya, J.
    Purification and properties of soluble and bound gamma-glutamyltransferases from radish cotyledon (2006), Biosci. Biotechnol. Biochem., 70, 369-376.
    View publication on PubMed
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine
-
 Raphanus sativus
acivicin
-
 Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
0.032
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
0.038
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
0.038
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
0.042
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
0.042
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
0.046
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
0.046
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
0.21
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
0.22
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
0.39
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
0.41
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
0.44
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
0.47
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
0.6
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
0.66
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.4
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
4.3
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
4.4
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall bound to, enzyme form GGT A Raphanus sativus 5618
-
cell wall bound to, enzyme form GGT B Raphanus sativus 5618
-
soluble enzyme form GGT I Raphanus sativus
-
-
soluble enzyme form GGT II Raphanus sativus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
21000
-
 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
42000
-
 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
42000
-
 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
61000
-
 enzyme form GGT A, gel filtration Raphanus sativus
61000
-
 enzyme form GGT B, gel filtration Raphanus sativus
63000
-
 enzyme form GGT I, gel filtration Raphanus sativus
63000
-
 enzyme form GGT II, gel filtration Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme form GGT A Raphanus sativus
glycoprotein enzyme form GGT B Raphanus sativus
glycoprotein enzyme form GGT I Raphanus sativus
glycoprotein enzyme form GGT II Raphanus sativus

Purification (Commentary)

Purification (Comment) Organism
enzyme form GGT A Raphanus sativus
enzyme form GGT B Raphanus sativus
enzyme form GGT I Raphanus sativus
enzyme form GGT II Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.59
-
 enzyme form GGT I Raphanus sativus
0.59
-
 enzyme form GGT II Raphanus sativus
1.48
-
 enzyme form GGT A Raphanus sativus
1.73
-
 enzyme form GGT B Raphanus sativus

Storage Stability

Storage Stability Organism
-20°C or 4°C, activity of enzyme form GGT A is lost within 1 week Raphanus sativus
-20°C or 4°C, activity of enzyme form GGT B is lost within 1 week Raphanus sativus
-80°C, stable, enzyme form GGT I Raphanus sativus
-80°C, stable, enzyme form GGT II Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 161% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 176% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 8% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 8% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
5-L-glutamyl-4-nitroanilide + Arg 124% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Arg 169% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Arg 44% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Arg 44% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 13% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 13% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 140% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 144% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
5-L-glutamyl-4-nitroanilide + Cys-Gly 15% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Cys-Gly 16% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Cys-Gly 36% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Cys-Gly 36% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Glu 11% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 14% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 14% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 43% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 48% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Met 137% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
5-L-glutamyl-4-nitroanilide + Met 141% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
5-L-glutamyl-4-nitroanilide + Met 43% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
5-L-glutamyl-4-nitroanilide + Met 43% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
gamma-Glu-Cys + Gly-Gly
-
 Raphanus sativus Cys + 5-L-glutamyl-Gly-Gly
-
 ?
GS-bimane + Gly-Gly GS-bimane is the conjugate of GSH and monobromobimane Raphanus sativus ?
-
 ?
GSH + Gly-Gly
-
 Raphanus sativus ?
-
 ?
GSSG + Gly-Gly
-
 Raphanus sativus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
dimer 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
monomer 1 * 61000, enzyme form GGT A, SDS-PAGE Raphanus sativus
monomer 1 * 61000, enzyme form GGT B, SDS-PAGE Raphanus sativus

Synonyms

Synonyms Comment Organism
GGT A
-
 Raphanus sativus
GGT I
-
 Raphanus sativus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
12
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
13
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
13
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
15
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
16
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
20
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
21
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
23
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
27
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
30
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
30
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
31
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
31
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
32
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
32
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
33
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
38
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
39
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
40
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzyme form GGT I Raphanus sativus
7.5
-
 enzyme form GGT II Raphanus sativus
7.5 8 enzyme form GGT A Raphanus sativus
7.5 8 enzyme form GGT B Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT A Raphanus sativus
6 9 pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT B Raphanus sativus
7 9 pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT I Raphanus sativus
7 9 pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT II Raphanus sativus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.016
-
 acivicin 37°C, pH 8.0, enzyme form GGT II Raphanus sativus
0.017
-
 acivicin 37°C, pH 8.0, enzyme form GGT I Raphanus sativus
0.081
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT I Raphanus sativus
0.085
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT II Raphanus sativus
0.096
-
 acivicin 37°C, pH 8.0, enzyme form GGT A Raphanus sativus
0.103
-
 acivicin 37°C, pH 8.0, enzyme form GGT B Raphanus sativus
0.47
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT B Raphanus sativus
0.57
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT A Raphanus sativus