Application | Comment | Organism |
---|---|---|
nutrition | the enzyme is suitable for food fermentation under high salt conditions, e.g. the fermentation of soy sauce and miso | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
GGT of Bacillus subtilis is synthesized only during the mid-stationary phase, which is inconvenient for industrial use, a strain overexpressing GGT for a sufficiently long period is generated to obtain large quantities of GGT. A plasmid vector, pHY300PLK, containing the ggt gene cloned from chromosomal DNA is introduced into a spo0A abrB double mutant strain, in which the level of GGT activity is high after the mid-stationary phase. The level of GGT activity in this strain increases steadily after the exponential phase, becoming 15fold higher than that in the parental strain | Bacillus subtilis |
General Stability | Organism |
---|---|
the enzyme is highly salt-tolerant, at pH 5.5 in presence of 18% NaCl, 49% of the transpeptidation activity and 86% of the hydrolysis activity remains, conversion of Gln to Glu shows 76% residual activity | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Bacillus subtilis | |
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 45000 + 1 * 21000, SDS-PAGE | Bacillus subtilis |
45000 | - |
1 * 45000 + 1 * 21000, SDS-PAGE | Bacillus subtilis |
63000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
168 | - |
Bacillus subtilis 168 | - |
168 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | in wild-type the enzyme is synthesized only during the mid-stationary phase | Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.49 | - |
hydrolysis | Bacillus subtilis |
100 | - |
transpeptidation | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly | 37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis 168 | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus subtilis 168 | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | - |
Bacillus subtilis | 4-nitroaniline + Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | - |
Bacillus subtilis 168 | 4-nitroaniline + Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Ala | 9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Ala | 9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis 168 | 4-nitroaniline + 5-L-glutamyl-L-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Arg | 21.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Asn | 24.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Asn | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Asp | 20.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Cys | 11.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Gln | 5.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Gln | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Glu | 8.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + L-His | 35.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-His | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Ile | 19.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Ile | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Leu | 21.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Leu | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Lys | 11.2% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Lys | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Met | 50.6% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Phe | 38.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Phe | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Pro | 12.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Pro | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Ser | 12.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Ser | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Thr | 34% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Thr | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Trp | 30.9% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Trp | - |
? | |
5-L-glutamyl-4-nitroanilide + L-Val | 30.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-L-Val | - |
? | |
5-L-glutamyl-4-nitroanilide + taurine | 68.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | 4-nitroaniline + 5-L-glutamyl-taurine | - |
? | |
5-L-glutamyl-L-His + Gly-Gly | 106% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-His + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-L-Met + Gly-Gly | 114% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-Met + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-L-Phe + Gly-Gly | 103% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-Phe + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-L-Trp + Gly-Gly | 127% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-Trp + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-L-Tyr + Gly-Gly | 94.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-Tyr + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-L-Val + Gly-Gly | 113% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | L-Val + 5-L-glutamyl-Gly-Gly | - |
? | |
Gln + H2O | - |
Bacillus subtilis | Glu + NH3 | - |
? | |
Gln + H2O | - |
Bacillus subtilis 168 | Glu + NH3 | - |
? | |
GSH + Gly-Gly | 98.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | ? | - |
? | |
L-Gln + Gly-Gly | 78.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly | Bacillus subtilis | NH3 + 5-L-glutamyl-Gly-Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 45000 + 1 * 21000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
GGT | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 8.7, 15 min, stable up to | Bacillus subtilis |
50 | - |
pH 8.7, 15 min, 44% inactivation | Bacillus subtilis |
60 | - |
pH 8.7, 15 min, complete inactivation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | glutaminase activity | Bacillus subtilis |
8.5 | 11 | hydrolysis of 5-L-glutamyl-4-nitroanilide | Bacillus subtilis |
9 | 9.5 | transpeptidation with 5-L-glutamyl-4-nitroanilide and glycylglycine | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | pH 8.0: about 60% of maximal activity, pH 11: about 75% of maximal activity, transpeptidation with 5-L-glutamyl-4-nitroanilide and glycylglycine | Bacillus subtilis |
8 | 11 | pH 8.0: about 60% of maximal activity, pH 8.5-11: optimum, hydrolysis of 5-L-glutamyl-4-nitroanilide | Bacillus subtilis |