Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + L-methioninamide | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methioninamide | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionyl(2,2,2-trifluoroethylamide) | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionyl(2,2,2-trifluoroethylamide) | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionyl(2-fluoroethylamide) | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionyl(2-fluoroethylamide) | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionyl(3,3,3-trifluoropropylamide) | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionyl(3,3,3-trifluoropropylamide) | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionylethylamide | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionylethylamide | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionylpropylamide | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionylpropylamide | - |
? | |
additional information | the mechanism of the deacylation step of GGT-mediated transpeptidation using L-methionine derivatives as acceptor substrates is consistent with the observed Bronsted linear free energy relationship, pH-rate profile, and solvent kinetic isotope effects. It is proposed that in the rate-limiting step these acceptor substrates carry out a nucleophilic attack on the acyl-enzyme intermediate, with simultaneous deprotonation of the nucleophilic amino group, to form a tetrahedral intermediate. At the transition state, nucleophilic attack is more advanced than deprotonation of the amine, leading to significant development of positive charge on the nucleophilic nitrogen | Rattus norvegicus | ? | - |
? |