Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Ikeda, Y.; Fujii, J.; Taniguchi, N.
    Effects of substitutions of the conserved histidine residues in human gamma-glutamyl transpeptidase (1996), J. Biochem., 119, 1166-1170.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant wild-type and mutants in Spodoptera frugiperda Sf21 cells via baculovirus infection Homo sapiens

Protein Variants

Protein Variants Comment Organism
H383A site-directed mutagenesis of conserved His383 residue, 3fold reduced activity and 62% reduced Vmax, altered binding of acceptor Homo sapiens
H383A/H505A site-directed mutagenesis, 37fold reduced activity Homo sapiens
H505A site-directed mutagenesis, 10fold reduced activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Homo sapiens
0.008
-
L-Glu-4-nitroanilide hydrolase reaction Homo sapiens
1.4
-
L-Glu-4-nitroanilide recombinant wild-type, transpeptidation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants from Sf21 insect cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
purified recombinant mutant H383A/H505A Homo sapiens
47
-
purified recombinant mutant H505A Homo sapiens
150
-
purified recombinant mutant H383A Homo sapiens
440
-
purified recombinant wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
Homo sapiens peptide + 5-L-glutamyl amino acid
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
?
5-L-glutamyl-4-nitroanilide + H2O hydrolase reaction Homo sapiens 4-nitroaniline + L-glutamate
-
?
L-Glu-4-nitroanilide + glutathione
-
Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens