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Literature summary for 2.3.2.2 extracted from
- Expression of an active glycosylated human gamma-glutamyl transpeptidase mutant that lacks a membrane anchor domain (1995), Proc. Natl. Acad. Sci. USA, 92, 126-130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant lacking the putative signal sequence/anchor domain, amino acid 1-27, in Spodoptera frugiperda Sf21 cells via baculovirus infection | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| brefeldin A | inhibition of recombinant mutant enzyme secretion into cell culture medium from Sf21 cells, accumulation in the cells | Homo sapiens |  |
| Urea | complete inactivation of wild-type and mutant at 6 M, low activity at 4 M | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
L-Glu-4-nitroanilide | recombinant wild-type | Homo sapiens | |
| 2.1 | - |
L-Glu-4-nitroanilide | recombinant mutant enzyme | Homo sapiens | |
| 2.9 | 2.96 | glycylglycine | recombinant wild-type enzyme | Homo sapiens | |
| 3.4 | - |
glycylglycine | recombinant mutant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | mutant lacking the membrane anchor domain, secretion into the medium of recombinant Sf21 cell culture | Homo sapiens | - |
- |
| membrane | wild-type, membrane-bound | Homo sapiens | 16020 | - |
| microsome | recombinant wild-type in Sf21 cells | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 43000-44000 + x * 24000, wild-type and mutant enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant wild-type and mutant | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutants from Sf21 insect cells | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 440 | - |
purified recombinant wild-type enzyme | Homo sapiens |
| 450 | - |
purified recombinant mutant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5-L-glutamyl)-peptide + acceptor + H+ | - |
Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
| L-Glu-4-nitroanilide + glutathione | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000-44000 + x * 24000, wild-type and mutant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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