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Literature summary for 2.3.2.2 extracted from

  • Iwami, K.; Yasumoto, K.; Nakamura, K.; Mitsuda, H.
    Properties of gamma-glutamyltransferase from Lentinus edodes (1975), Agric. Biol. Chem., 39, 1933-1940.
No PubMed abstract available
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no inhibition Lentinula edodes
Cu2+ strong Lentinula edodes
EDTA no inhibition Lentinula edodes
Hg2+ strong Lentinula edodes
L- or D-Serine/borate 1:1 mixture, strong Lentinula edodes
Mg2+ no inhibition Lentinula edodes
additional information no inhibition by arsenite, citrate, cyanide and borate Lentinula edodes
p-chloromercuribenzoate strong Lentinula edodes
phosphate
-
 Lentinula edodes
Zn2+ strong Lentinula edodes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 L-Glu-4-nitroanilide
-
 Lentinula edodes

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Lentinula edodes
-
-

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
 shiitake mushroom
-

Purification (Commentary)

Purification (Comment) Organism
partial Lentinula edodes

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Lentinula edodes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Lentinula edodes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Lentinula edodes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Lentinula edodes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Lentinula edodes peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate alpha-enantiomer of aminobutyrate Lentinula edodes 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + glycine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-alanine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-arginine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
5-L-glutamyl-4-nitroanilide + L-aspartate
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-cysteine
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + L-histidine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
-
 ?
5-L-glutamyl-4-nitroanilide + L-leucine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-lysine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-proline
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
5-L-glutamyl-4-nitroanilide + L-serine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
5-L-glutamyl-4-nitroanilide + L-threonine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
5-L-glutamyl-4-nitroanilide + L-tryptophan
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
5-L-glutamyl-4-nitroanilide + L-valine
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
5-L-glutamyl-4-nitroanilide + S-alkylcysteine sulfoxide best substrates Lentinula edodes 4-nitroaniline + 5-L-glutamyl-S-alkylcysteine sulfoxide
-
 ?
glutathione + acceptor acceptor: hydroxylamine Lentinula edodes cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Lentinula edodes 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Lentinula edodes
65
-
 inactivation, GSH and serine/borate protect Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Lentinula edodes

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 about half-maximal activity at pH 6.5 and 9.5 Lentinula edodes