Crystallization (Comment) | Organism |
---|---|
ammonium sulfate precipitation, refrigerator, 2 weeks | Proteus mirabilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Proteus mirabilis | |
Aminooxyacetate | hydrolase reaction | Proteus mirabilis | |
beta-chloro-L-alanine | - |
Proteus mirabilis | |
glutathione | no inhibition | Proteus mirabilis | |
glycine | weak | Proteus mirabilis | |
Hg2+ | - |
Proteus mirabilis | |
iodoacetamide | - |
Proteus mirabilis | |
iodoacetate | weak | Proteus mirabilis | |
L- or D-Serine/borate | L-serine/borate, not D-serine/borate | Proteus mirabilis | |
L-alanine | - |
Proteus mirabilis | |
L-glutamine | not D- | Proteus mirabilis | |
additional information | inhibition by diverse amino acids, overview | Proteus mirabilis | |
N-ethylmaleimide | no inhibition | Proteus mirabilis | |
p-chloromercuribenzoate | no inhibition | Proteus mirabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
glutathione | hydrolase reaction | Proteus mirabilis | |
0.13 | 0.18 | L-Glu-4-nitroanilide | with GSH | Proteus mirabilis | |
0.18 | - |
glutathione | transpeptidation | Proteus mirabilis | |
0.31 | - |
L-Glu-4-nitroanilide | hydrolase reaction | Proteus mirabilis | |
0.4 | - |
L-Glu-4-nitroanilide | transpeptidation | Proteus mirabilis | |
2.9 | 2.96 | glycylglycine | - |
Proteus mirabilis | |
10 | - |
L-glutamine | hydrolase reaction | Proteus mirabilis | |
32 | - |
L-Phe | - |
Proteus mirabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 47000 + 1 * 28000, SDS-PAGE | Proteus mirabilis |
47000 | - |
1 * 47000 + 1 * 28000, SDS-PAGE | Proteus mirabilis |
80000 | - |
gel filtration | Proteus mirabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Proteus mirabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
purified enzyme | Proteus mirabilis |
Storage Stability | Organism |
---|---|
4°C, crystalline, at least 3 months | Proteus mirabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Proteus mirabilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptors are amino acids or dipeptides | Proteus mirabilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: autotranspeptidation with another donor molecule as acceptor | Proteus mirabilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Proteus mirabilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Proteus mirabilis | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Proteus mirabilis | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
GSH + L-glutamine | - |
Proteus mirabilis | ? | - |
? | |
GSH + L-Phe | - |
Proteus mirabilis | ? | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Proteus mirabilis | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 47000 + 1 * 28000, SDS-PAGE | Proteus mirabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Proteus mirabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10% loss of activity within 10 min | Proteus mirabilis |
45 | - |
37% loss of activity within 10 min | Proteus mirabilis |
50 | - |
95% loss of activity within 10 min | Proteus mirabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
hydrolase reaction | Proteus mirabilis |
7.5 | 8 | transpeptidation | Proteus mirabilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
50% loss of activity after 60 min, 37°C | Proteus mirabilis |
5 | 9 | at least 60 min stable, 37°C | Proteus mirabilis |
10 | - |
50% loss of activity after 60 min, 37°C | Proteus mirabilis |