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Literature summary for 2.3.2.2 extracted from

  • Allison, D.
    gamma-Glutamyl transpeptidase: kinetics and mechanism (1985), Methods Enzymol., 113, 419-437.
    View publication on PubMed
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
hippurate stimulates hydrolase reaction, inhibits transpeptidation Mammalia
hippurate stimulates hydrolase reaction, inhibits transpeptidation Mus musculus
hippurate stimulates hydrolase reaction, inhibits transpeptidation Rattus norvegicus
hippurate stimulates hydrolase reaction, inhibits transpeptidation Ovis aries
Maleate
-
 Mammalia
Maleate
-
 Mus musculus
Maleate
-
 Ovis aries
Maleate stimulates hydrolase activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) no inhibition Mammalia
5,5'-dithiobis(2-nitrobenzoate) no inhibition Ovis aries
5,5'-dithiobis(2-nitrobenzoate) no inhibition Rattus norvegicus
5-glutamylhydrazones
-
 Mammalia
5-glutamylhydrazones
-
 Mus musculus
5-glutamylhydrazones
-
 Ovis aries
5-glutamylhydrazones
-
 Rattus norvegicus
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Mammalia
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Mus musculus
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Ovis aries
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Rattus norvegicus
6-diazo-5-oxo-L-norleucine
-
 Mammalia
6-diazo-5-oxo-L-norleucine
-
 Mus musculus
6-diazo-5-oxo-L-norleucine
-
 Ovis aries
6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
Acetazolamide Maleate
-
 Mammalia
Acetazolamide Maleate
-
 Mus musculus
Acetazolamide Maleate
-
 Ovis aries
Acetazolamide Maleate
-
 Rattus norvegicus
Bromocresol green
-
 Mammalia
Bromocresol green
-
 Mus musculus
Bromocresol green
-
 Ovis aries
Bromocresol green
-
 Rattus norvegicus
iodoacetamide irreversible Mammalia
iodoacetamide
-
 Mus musculus
iodoacetamide irreversible Ovis aries
iodoacetamide irreversible Rattus norvegicus
L- or D-Serine/borate formation of a tetrahedral complex at the active site Mammalia
L- or D-Serine/borate
-
 Mus musculus
L- or D-Serine/borate formation of a tetrahedral complex at the active site Ovis aries
L- or D-Serine/borate formation of a tetrahedral complex at the active site Rattus norvegicus
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Mammalia
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Mus musculus
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Ovis aries
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Rattus norvegicus
L-azaserine
-
 Mammalia
L-azaserine
-
 Mus musculus
L-azaserine
-
 Ovis aries
L-azaserine
-
 Rattus norvegicus
additional information
-
 Mammalia
additional information
-
 Mus musculus
additional information
-
 Ovis aries
additional information inhibition by diverse amino acids, overview Rattus norvegicus
N-ethylmaleimide weak Mammalia
N-ethylmaleimide weak Ovis aries
N-ethylmaleimide weak Rattus norvegicus
p-chloromercuribenzoate no inhibition Mammalia
p-chloromercuribenzoate no inhibition Ovis aries
p-chloromercuribenzoate no inhibition Rattus norvegicus
phenylhydrazides
-
 Mammalia
phenylhydrazides
-
 Mus musculus
phenylhydrazides
-
 Ovis aries
phenylhydrazides
-
 Rattus norvegicus
Sulfobromophthalein derivatives
-
 Mammalia
Sulfobromophthalein derivatives
-
 Mus musculus
Sulfobromophthalein derivatives
-
 Ovis aries
Sulfobromophthalein derivatives
-
 Rattus norvegicus
Sulfophthalein derivatives
-
 Mammalia
Sulfophthalein derivatives
-
 Mus musculus
Sulfophthalein derivatives
-
 Ovis aries
Sulfophthalein derivatives
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mammalia
additional information
-
 additional information
-
 Mus musculus
additional information
-
 additional information kinetic study Rattus norvegicus
additional information
-
 additional information kinetic study Ovis aries
additional information
-
 additional information Km values for several amino acids and dipeptides, overview Rattus norvegicus
0.005 0.0068 L-Glu-4-nitroanilide
-
 Rattus norvegicus
0.029 0.035 L-cystine kidney enzyme Rattus norvegicus
0.031
-
 5-D-Glu-4-nitroanilide
-
 Rattus norvegicus
0.13 0.18 L-Glu-4-nitroanilide with L-Cys-bis-glycine, rat kidney enzyme Rattus norvegicus
0.21 0.32 L-Glu-4-nitroanilide with L-Met-Gly, kidney enzyme Rattus norvegicus
0.76
-
 L-Gln rat kidney enzyme Rattus norvegicus
1.09
-
 L-cysteinylglycine
-
 Rattus norvegicus
1.09
-
 L-cysteinylglycine L-Cys-Gly, rat kidney enzyme Rattus norvegicus
2.9 2.96 glycylglycine rat kidney enzyme Rattus norvegicus
4 4.7 glycylglycine L-Met, rat kidney enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ Mammalia involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Mus musculus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Rattus norvegicus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Ovis aries involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Mammalia concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Rattus norvegicus concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Ovis aries concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?

Organism

Organism UniProt Comment Textmining
Mammalia
-
-
-
Mus musculus
-
-
-
Ovis aries
-
-
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mammalia
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mus musculus
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Rattus norvegicus
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Ovis aries
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Rattus norvegicus
a
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ovis aries
a

Source Tissue

Source Tissue Comment Organism Textmining
cerebellum
-
 Mus musculus
-
kidney
-
 Mammalia
-
kidney
-
 Mus musculus
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities with diverse substrates Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ imino acids are no substrates Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
5-D-Glu-4-nitroanilide + an amino acid
-
 Rattus norvegicus ?
-
 ?
5-D-glutamyl-4-nitroanilide + acceptor
-
 Rattus norvegicus 4-nitroaniline + 5-D-glutamyl-acceptor
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Mammalia 4-nitroaniline + glutathione
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Mus musculus 4-nitroaniline + glutathione
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Rattus norvegicus 4-nitroaniline + glutathione
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Ovis aries 4-nitroaniline + glutathione
-
 ?
5-L-glutamyl-4-nitroanilide + L-cystine best acceptor Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
a (5-L-glutamyl)-peptide + L-cysteinylglycine
-
 Rattus norvegicus ?
-
 ?
glutathione + acceptor
-
 Mammalia cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
glutathione + acceptor
-
 Mus musculus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
glutathione + acceptor
-
 Rattus norvegicus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
glutathione + acceptor
-
 Ovis aries cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
glutathione + glutathione autotranspeptidation Mammalia L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
glutathione + glutathione autotranspeptidation Mus musculus L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
glutathione + glutathione autotranspeptidation Rattus norvegicus L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
glutathione + glutathione autotranspeptidation Ovis aries L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
glutathione + L-cysteinylglycine
-
 Mammalia L-cysteinylglycine + glutathione
-
 ?
glutathione + L-cysteinylglycine
-
 Mus musculus L-cysteinylglycine + glutathione
-
 ?
glutathione + L-cysteinylglycine
-
 Rattus norvegicus L-cysteinylglycine + glutathione
-
 ?
glutathione + L-cysteinylglycine
-
 Ovis aries L-cysteinylglycine + glutathione
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?
additional information
-
 Rattus norvegicus ?
-
 ?
additional information the donor substrate determines the acceptor specificity Ovis aries ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Rattus norvegicus
37
-
 assay at Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information Ki values for diverse amino acids, overview Mammalia
additional information
-
 additional information Ki values for diverse amino acids, overview Mus musculus
additional information
-
 additional information Ki values for diverse amino acids, overview Rattus norvegicus