Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Free bile acids | activation | Rattus norvegicus | |
Free bile acids | activation | Sus scrofa | |
Maleate | - |
Rattus norvegicus | |
Maleate | - |
Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L- or D-Serine/borate | - |
Rattus norvegicus | |
L- or D-Serine/borate | transition-state inhibitor | Sus scrofa | |
L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid | i.e. acivicin; strong, irreversible, reacts with glutamyl binding site | Sus scrofa | |
Phenobarbital | - |
Rattus norvegicus | |
Phenobarbital | - |
Sus scrofa | |
Thiobarbituric acid | - |
Rattus norvegicus | |
Thiobarbituric acid | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Rattus norvegicus | |
additional information | - |
additional information | kinetic study | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
membrane | - |
Sus scrofa | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
papain | Rattus norvegicus |
Triton X-100 | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | mechanism | Rattus norvegicus | |
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | mechanism | Sus scrofa | |
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | kinetic study | Rattus norvegicus | |
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | kinetic study | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
792 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | donors: 5-ethyl-Glu and beta-benzyl-Glu, hydrolyzed by pig enzyme | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: leukotriene C | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: leukotriene C | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: glutathione disulfide | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: glutathione disulfide | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: S-acetophenoneglutathione | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: S-acetophenoneglutathione | Sus scrofa | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + L-cystine | best acceptor | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-cystine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-cystine | best acceptor | Sus scrofa | 4-nitroaniline + 5-L-glutamyl-L-cystine | - |
? | |
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine | - |
Rattus norvegicus | 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine | - |
? | |
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine | - |
Sus scrofa | 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine | - |
? | |
glutathione + acceptor | best donor | Rattus norvegicus | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
glutathione + acceptor | best donor | Sus scrofa | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
glutathione + leukotriene D | - |
Sus scrofa | cysteinylglycine + leukotriene C | - |
? | |
leukotriene C4 + acceptor | i.e. glutathione containing derivative of arachidonic acid, acceptors are H2O, amino acids or dipeptides | Rattus norvegicus | leukotriene D4 + glutamyl-acceptor | - |
? | |
leukotriene C4 + acceptor | i.e. glutathione containing derivative of arachidonic acid, acceptors are H2O, amino acids or dipeptides | Sus scrofa | leukotriene D4 + glutamyl-acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Sus scrofa |