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Literature summary for 2.3.2.2 extracted from
- Purification and some properties of gamma-glutamyl transpeptidase from azo dye-induced hepatoma (1974), J. Biochem., 75, 473-480.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | weak | Rattus norvegicus |  |
| EDTA | no inhibition | Rattus norvegicus | |
| iodoacetamide | no inhibition | Rattus norvegicus | |
| iodoacetate | no inhibition | Rattus norvegicus | |
| N-ethylmaleimide | weak | Rattus norvegicus | |
| p-chloromercuribenzoate | no inhibition | Rattus norvegicus | |
| Zn2+ | strong | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
L-Glu-4-nitroanilide | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation | Rattus norvegicus | |
| Ca2+ | activation, transpeptidase | Rattus norvegicus | |
| Ca2+ | not hydrolase | Rattus norvegicus | |
| Mg2+ | slight activation | Rattus norvegicus | |
| Mg2+ | activation, transpeptidase | Rattus norvegicus | |
| Mg2+ | not hydrolase | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Rattus norvegicus |
| 113000 | - |
analytical ultracentrifugation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male, Donryu | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 36% carbohydrate | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized by deoxycholate | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatoma cell | azo dye-induced | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 181 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -22°C, at least 3 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is 5-L-Glu-4-nitroanilide | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
ir | |
| glutathione + acceptor | - |
Rattus norvegicus | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
| L-Glu-4-nitroanilide + glutathione | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
12 min | Rattus norvegicus |
| 58 | - |
in the presence of glutathione, 0.02 M, at least 60 min stable | Rattus norvegicus |
| 58 | - |
20% of initial activity retained after 60 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 3.0 | Rattus norvegicus |
| 8.8 | 9 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 9.6 | about 70% of maximal activity at pH 8.1, about 75% of maximal activity at pH 9.6 | Rattus norvegicus |
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