Cloned (Comment) | Organism |
---|---|
gene alr0975 or pcs, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha, and functional constitutive overexpression in Anabaena sp. strain PCC 7120, semiquantitative RT-PCR enzyme expression analysis | Nostoc sp. PCC 7120 = FACHB-418 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an extra copy of phytochelatin-encoding gene phytochelatin synthase (pcs) is constitutively expressed in Anabaena sp. strain PCC7120 (An7120), an integrative expression vector pFPN is used for incorporating a cassette of genes for genomic integration and expression. Comparative growth behavior of wild-type strain and overexpressed strain AnFPN-pcs under LC50 doses of UV-B, salt, heat, copper, carbofuran, and cadmium resulted in decrement in specific growth rate by 26, 21, 27, 13, 30, and 17%, respectively, in An7120 compared to AnFPN-pcs thereby suggesting that the transformed cyanobacterium AnFPN-pcs has developed tolerance and hence shows better growth performances under different abiotic stresses. Comparison of differentially expressed proteins in An7120 and AnFPN-pcs, and dynamics of differentially expressed proteins in An7120 and AnFPN-pcs, overview | Nostoc sp. PCC 7120 = FACHB-418 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Nostoc sp. PCC 7120 = FACHB-418 | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. PCC 7120 = FACHB-418 | Q8YY76 | SAG 25.82, UTEX 2576, i.e. Anabaena sp. PCC 7120 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Nostoc sp. PCC 7120 = FACHB-418 | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alr0975 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
PCS | - |
Nostoc sp. PCC 7120 = FACHB-418 |
phytochelatin synthase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nostoc sp. PCC 7120 = FACHB-418 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Nostoc sp. PCC 7120 = FACHB-418 |
General Information | Comment | Organism |
---|---|---|
physiological function | role of of enzyme PCS in abiotic stress tolerance | Nostoc sp. PCC 7120 = FACHB-418 |