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Literature summary for 2.3.2.15 extracted from

  • Vatamaniuk, O.K.; Mari, S.; Lang, A.; Chalasani, S.; Demkiv, L.O.; Rea, P.A.
    Phytochelatin synthase, a dipeptidyltransferase that undergoes multisite acylation with gamma-glutamylcysteine during catalysis: stoichiometric and site-directed mutagenic analysis of Arabidopsis thaliana PCS1-catalyzed phytochelatin synthesis (2004), J. Biol. Chem., 279, 22449-22460.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of AtPCS1-FLAG in Saccharomyces cerevisiae Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C109A mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C109S mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C113A mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C113S mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C56S mutation abolishes Cd2+ tolerance observed with wild-type enzyme, causes negligible intracellular phytochelatin accumulation Arabidopsis thaliana
C90A mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C90S mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C91A mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana
C91S mutant enzyme shows similar degree of Cd2+ tolerance on DTY167 cells as the wild-type equivalent Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n -Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n+1 -Gly
-
?

Synonyms

Synonyms Comment Organism
PCS1
-
Arabidopsis thaliana