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Literature summary for 2.3.2.14 extracted from
- Occurence and some properties of a novel gamma-glutamyltransferase responsible for the synthesis of gamma-L-glutamyl-D-alanine in pea seedlings (1982), Biochim. Biophys. Acta, 716, 194-200.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | - |
Pisum sativum |  |
| citrate | to some extent | Pisum sativum | |
| L-Serine borate | - |
Pisum sativum | |
| additional information | no inhibition by Ca2+, Mg2+, NH4+, and maleate | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
L-glutamine | - |
Pisum sativum | |
| 2.9 | - |
D-alanine | - |
Pisum sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
cv. Alaska | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | decotyledonized | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.245 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-L-glutamylethylester + D-alanine | equally effective as L-glutamine | Pisum sativum | ? | - |
? | |
| L-glutamine + D-2-amino-n-butanoic acid | - |
Pisum sativum | ? | - |
? | |
| L-glutamine + D-alanine | best substrate | Pisum sativum | NH3 + gamma-L-glutamyl-D-alanine | - |
? | |
| L-glutamine + D-phenylalanine | - |
Pisum sativum | NH3 + gamma-L-glutamyl-L-phenylalanine | - |
? | |
| additional information | no substrates are D-valine, D-leucine, D-aspartic acid, D-glutamic acid, D-serine, D-proline | Pisum sativum | ? | - |
? | |
| reduced glutathione + D-alanine | 50% as effective as L-glutamine | Pisum sativum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Pisum sativum |
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