Application | Comment | Organism |
---|---|---|
food industry | transglutaminase MTG-TX can be used in food-related applications in salty environment, e.g. bacon or seafood | Streptomyces mobaraensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Streptomyces mobaraensis | |
Fe3+ | strong inhibition | Streptomyces mobaraensis | |
additional information | isozyme MTG-TX is not inhibited by Li+, Na+, K+, Ca2+, Mg2+, Ba2+, Mn2+, or the inhibitor phenylmethanesulfonyl fluoride (PMSF) | Streptomyces mobaraensis | |
N-ethylmaleimide | strong inhibition | Streptomyces mobaraensis | |
Zn2+ | strong inhibition | Streptomyces mobaraensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.37 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis | |
23.12 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces mobaraensis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by Cu2+ and Fe3+ | Streptomyces mobaraensis | |
NaCl | purification of a high-salt resistent microbial transglutaminase, isozyme MTG-TX, 79.8% activity remaining | Streptomyces mobaraensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein glutamine + alkylamine | Streptomyces mobaraensis | - |
protein N5-alkylglutamine + NH3 | - |
? | |
protein glutamine + alkylamine | Streptomyces mobaraensis TX | - |
protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | P81453 | collected from soil | - |
Streptomyces mobaraensis TX | P81453 | collected from soil | - |
Purification (Comment) | Organism |
---|---|
purification of extracellular high-salt resistent microbial transglutaminase, isozyme MTG-TX, by ethanol precipitation, cation exchange chromatography, hydrophobic interaction chromatography, and gel filtration, method optimization, overview | Streptomyces mobaraensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein glutamine + alkylamine = protein N5-alkylglutamine + NH3 | ping-pong mechanism | Streptomyces mobaraensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.2 | - |
purified extracellular isozyme MTG-TX, pH 6.0, 37°C | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-CBZ-Glu-Gly + hydroxylamine | - |
Streptomyces mobaraensis | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
N-CBZ-Glu-Gly + hydroxylamine | - |
Streptomyces mobaraensis TX | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
protein glutamine + alkylamine | - |
Streptomyces mobaraensis | protein N5-alkylglutamine + NH3 | - |
? | |
protein glutamine + alkylamine | - |
Streptomyces mobaraensis TX | protein N5-alkylglutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37816, mass spectrometry | Streptomyces mobaraensis |
Synonyms | Comment | Organism |
---|---|---|
microbial transglutaminase | - |
Streptomyces mobaraensis |
MTG-TX | - |
Streptomyces mobaraensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
isozyme MTG-TX | Streptomyces mobaraensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile overview | Streptomyces mobaraensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability | Streptomyces mobaraensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis | |
1.83 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
isozyme MTG-TX | Streptomyces mobaraensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | activity range of isozyme MTG-TX | Streptomyces mobaraensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability | Streptomyces mobaraensis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme shows a ping-pong mechanism | Streptomyces mobaraensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis | |
1.34 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis |