Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chlorpyrifos | - |
Mythimna separata | |
spinosyn A | as spinosad, a mixture of spinosyn A und spinosyn D | Mythimna separata | |
spinosyn D | as spinosad, a mixture of spinosyn A und spinosyn D | Mythimna separata |
Application | Comment | Organism |
---|---|---|
agriculture | physiological role of MsTGase and the potential impact of its regulation on MsTGase-associated pest management | Mythimna separata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | significant inhibition | Mythimna separata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.83 | - |
N-Cbz-Gln-Gly | native enzyme, pH 7.5, 42°C | Mythimna separata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, activates | Mythimna separata | |
additional information | the enzyme does not possess metal ion at its catalytic active site | Mythimna separata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein glutamine + alkylamine | Mythimna separata | - |
protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mythimna separata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 48fold from larvae by gel filtration and anion exchange chromatography | Mythimna separata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Mythimna separata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bovine serum albumin glutamine + alkylamine | - |
Mythimna separata | bovine serum albumin N5-alkylglutamine + NH3 | - |
? | |
casein glutamine + alkylamine | - |
Mythimna separata | casein N5-alkylglutamine + NH3 | - |
? | |
additional information | the formation of intermolecular cross-links of casein and bovine serum albumin polymerized by MsTGase in the presence of DTT is observed | Mythimna separata | ? | - |
- |
|
N-CBZ-Gln-Gly + hydroxylamine | - |
Mythimna separata | L-CBZ-Gln(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
protein glutamine + alkylamine | - |
Mythimna separata | protein N5-alkylglutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63500, SDS-PAGE | Mythimna separata |
Synonyms | Comment | Organism |
---|---|---|
MsTGase | - |
Mythimna separata |
transglutaminase | - |
Mythimna separata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Mythimna separata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mythimna separata |
General Information | Comment | Organism |
---|---|---|
additional information | the N-terminal amino acid sequence of MsTGase is GKIEEG-LVI | Mythimna separata |