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Literature summary for 2.3.2.13 extracted from

  • Zhang, L.; Rao, W.; Muhayimana, S.; Zhang, X.; Xu, J.; Xiao, C.; Huang, Q.
    Purification and biochemical characterization of a novel transglutaminase from Mythimna separata larvae (Noctuidae, Lepidoptera) (2018), J. Biotechnol., 265, 1-7 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
chlorpyrifos
-
Mythimna separata
spinosyn A as spinosad, a mixture of spinosyn A und spinosyn D Mythimna separata
spinosyn D as spinosad, a mixture of spinosyn A und spinosyn D Mythimna separata

Application

Application Comment Organism
agriculture physiological role of MsTGase and the potential impact of its regulation on MsTGase-associated pest management Mythimna separata

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ significant inhibition Mythimna separata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.83
-
N-Cbz-Gln-Gly native enzyme, pH 7.5, 42°C Mythimna separata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, activates Mythimna separata
additional information the enzyme does not possess metal ion at its catalytic active site Mythimna separata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein glutamine + alkylamine Mythimna separata
-
protein N5-alkylglutamine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Mythimna separata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 48fold from larvae by gel filtration and anion exchange chromatography Mythimna separata

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Mythimna separata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bovine serum albumin glutamine + alkylamine
-
Mythimna separata bovine serum albumin N5-alkylglutamine + NH3
-
?
casein glutamine + alkylamine
-
Mythimna separata casein N5-alkylglutamine + NH3
-
?
additional information the formation of intermolecular cross-links of casein and bovine serum albumin polymerized by MsTGase in the presence of DTT is observed Mythimna separata ?
-
-
N-CBZ-Gln-Gly + hydroxylamine
-
Mythimna separata L-CBZ-Gln(gamma-monohydroxamate)-Gly + NH3
-
?
protein glutamine + alkylamine
-
Mythimna separata protein N5-alkylglutamine + NH3
-
?

Subunits

Subunits Comment Organism
? x * 63500, SDS-PAGE Mythimna separata

Synonyms

Synonyms Comment Organism
MsTGase
-
Mythimna separata
transglutaminase
-
Mythimna separata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Mythimna separata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mythimna separata

General Information

General Information Comment Organism
additional information the N-terminal amino acid sequence of MsTGase is GKIEEG-LVI Mythimna separata