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Literature summary for 2.3.2.13 extracted from

  • Li, H.; Cui, Y.; Zhang, L.; Zhang, L.; Liu, H.; Yu, J.
    Optimization of recombinant Zea mays transglutaminase production and its influence on the functional properties of yogurt (2017), Food Sci. Biotechnol., 26, 723-730 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Application

Application Comment Organism
food industry recombinant enzyme TGZo from Zea mays is suitable for food processing. Cross-linking in proteins by TGase leads to the change of protein functionalities such as water-holding capacity, solubility, gelation property, emulsifying capacity, and nutritional value. TGZo from Zea mays is tested for cow milk yoghurt production, method evaluation, overview. Plackett-Burman design and response surface methodolog. Texture analysis of cow milk yogurts cross-linked by different concentrations of TGZo Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of the enzyme in Pichia pastoris strain GS115 Zea mays

Protein Variants

Protein Variants Comment Organism
additional information production of optimized Zea mays transglutaminase (TGZo) using Pichia pastoris strain GS115 (pPIC9K-tgzo), method optimization, overview Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein glutamine + alkylamine Zea mays
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-CBZ-Gln-Gly + hydroxylamine
-
 Zea mays ?
-
 ?
protein glutamine + alkylamine
-
 Zea mays protein N5-alkylglutamine + NH3
-
 ?

Synonyms

Synonyms Comment Organism
TGase
-
 Zea mays
TGZo
-
 Zea mays
transglutaminase
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Zea mays

General Information

General Information Comment Organism
additional information comparison of the recombinantly expressed enzyme TGZo from Zea mays with the microbial transglutaminase(MTG) enzymes from Streptomyces mobaraensis and Streptomyces hygroscopicus. TGZo has higher thermostability and wider range of pH than the MTGs from the Streptomyces strains. Texture analysis of cow milk yogurts cross-linked by different concentrations of TGZo and MTG Zea mays
physiological function transglutaminase is a transferase that catalyzes the acyl transfer reaction between the gamma-carboxamide group in glutamine residues and various primary amines in polyamine Zea mays