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Literature summary for 2.3.2.13 extracted from
- Purification of bovine liver transglutaminase by gel filtration (2020), Appl. Biol. Chem., 63, 6 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 20% inhibition at 15 mM | Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required, best activating at 6 mM | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Bos taurus | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from liver by gel filtration and ultrafiltration | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
purified enzyme, pH 6.0, 37°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-CBZ-Glu-Gly + hydroxylamine | - |
Bos taurus | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Bos taurus | protein N5-alkylglutamine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGase | - |
Bos taurus |
| transglutaminase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 65 | activity range, over 40% of maximal activity at 30-60°C | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | purified enzyme, stable at for at least 60 min | Bos taurus |
| 55 | - |
purified enzyme, loss of 40% activity after 60 min | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | the purified enzyme is stable between pH 6.5 and 8.0 when incubated for 30 min at 35°C. 70% residual activity above pH 7.0, loss of activity below pH 4.0 and above pH 8.5 | Bos taurus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | transglutaminases (TGase) catalyze the acyl transfer reaction between a free amine group (e.g. in a protein or peptide-bound lysine or an amine) and the gamma-carboxy amide group of proteins or peptide bound glutamine thus leading to the modification of proteins. When an epsilon-amino group of a peptide bound lysine acts as a acyl acceptor, isopeptide bond is formed between the glutamine and lysine residues in them, introducing both inter- and intramolecular covalent cross-links, resulting in the polymerization of the proteins | Bos taurus |
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Release 2023.1 (January 2023)
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