Literature summary for 2.3.2.13 extracted from

Rickert, M.; Strop, P.; Lui, V.; Melton-Witt, J.; Farias, S.E.; Foletti, D.; Shelton, D.; Pons, J.; Rajpal, A.
Production of soluble and active microbial transglutaminase in Escherichia coli for site-specific antibody drug conjugation (2016), Protein Sci., 25, 442-455.

Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces mobaraensis

Protein Variants

Protein Variants	Comment	Organism
D20A	the mutant shows reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
E29A	the mutant shows about 60% reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
I24A	the mutant shows reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
L16A	the mutant shows wild type activity	Streptomyces mobaraensis
L27A	the mutant shows about 40% reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
N23A	the mutant shows about 50% reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
N25A	the mutant shows reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
N28A	the mutant shows about 45% reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
V21A	the mutant shows about wild type activity	Streptomyces mobaraensis
Y10A	the mutant shows reduced activity compared to the wild type enzyme	Streptomyces mobaraensis
Y14A	the mutant shows wild type activity	Streptomyces mobaraensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39198	-	x * 39198, mass spectrometry	Streptomyces mobaraensis
39200	-	x * 39200, calculated from amino acid sequence	Streptomyces mobaraensis
40000	-	x * 40000, SDS-PAGE	Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein]-L-glutamine + alkylamine	Streptomyces mobaraensis	-	[protein]-N5-alkyl-L-glutamine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces mobaraensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Gln-Gly + alkylamine	-	Streptomyces mobaraensis	?	-	?
[protein]-L-glutamine + alkylamine	-	Streptomyces mobaraensis	[protein]-N5-alkyl-L-glutamine + NH3	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Streptomyces mobaraensis
?	x * 39198, mass spectrometry	Streptomyces mobaraensis
?	x * 39200, calculated from amino acid sequence	Streptomyces mobaraensis

Synonyms

Synonyms	Comment	Organism
microbial transglutaminase	-	Streptomyces mobaraensis
MTGase	-	Streptomyces mobaraensis
protein-glutamine gamma-glutamyltransferase	-	Streptomyces mobaraensis

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Release 2023.1 (January 2023)