Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces mobaraensis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 30% (w/v) PEG 8000, 50 mM NaCl, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.01% (w/v) NaN3, 100 mM cacodylic acid (pH 5.0), and 2% (v/v) glycerol | Streptomyces mobaraensis |
Protein Variants | Comment | Organism |
---|---|---|
D301A | the mutation drastically reduces the catalytic activity of the enzyme | Streptomyces mobaraensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GLGQGETYR | - |
Streptomyces mobaraensis | |
SQAETQR | - |
Streptomyces mobaraensis | |
SQAETYR | noncompetitive inhibition | Streptomyces mobaraensis | |
SYAETYR | noncompetitive inhibition | Streptomyces mobaraensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.66 | - |
Nalpha-benzyloxycarbonyl-L-glutaminylglycine | in 250 mM Tris acetate buffer (pH 8.0), at 37°C | Streptomyces mobaraensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine | Streptomyces mobaraensis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine | - |
Streptomyces mobaraensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTGase | - |
Streptomyces mobaraensis |
TGase | - |
Streptomyces mobaraensis |
transglutaminase | - |
Streptomyces mobaraensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.48 | - |
Nalpha-benzyloxycarbonyl-L-glutaminylglycine | in 250 mM Tris acetate buffer (pH 8.0), at 37°C | Streptomyces mobaraensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.65 | - |
in 250 mM Tris acetate buffer (pH 8.0), at 37°C | Streptomyces mobaraensis | SQAETYR | |
0.75 | - |
in 250 mM Tris acetate buffer (pH 8.0), at 37°C | Streptomyces mobaraensis | SYAETYR |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
Nalpha-benzyloxycarbonyl-L-glutaminylglycine | in 250 mM Tris acetate buffer (pH 8.0), at 37°C | Streptomyces mobaraensis |