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Literature summary for 2.3.2.13 extracted from

  • Stamnaes, J.; Pinkas, D.M.; Fleckenstein, B.; Khosla, C.; Sollid, L.M.
    Redox regulation of transglutaminase 2 activity (2010), J. Biol. Chem., 285, 25402-25409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C230A the mutant shows about wild type activity but is less susceptible to oxidation than the wild type enzyme Homo sapiens
C277S active-site mutant Homo sapiens
C370A the kcat/Km is 33% of the wild type enzyme Homo sapiens
C371A the kcat/Km is 5% of the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GSSG reversible inactivation, activity can be restored by treatment with dithiothreitol Homo sapiens
N-acetyl-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 active-site inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.2
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
11.5
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
30
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
30
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for catalytic activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is reversibly inactivated by oxidation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibronectin-bound gamma-glutamine + 5-biotinamidopentylamine
-
Homo sapiens fibronectin N5-biotinamidopentylglutamine + NH3
-
?
Nalpha-benzyloxycarbonyl-L-Gln-Gly + alkylamine
-
Homo sapiens ?
-
?
PQPQLPYPQPQLPY-NH2 + 5-biotinamidopentylamine
-
Homo sapiens ?
-
?
QLQPFPQPQLPY + 5-biotinamidopentylamine
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TG2
-
Homo sapiens
transglutaminase 2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
0.19
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00083
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
0.0055
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
0.015
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
0.017
-
Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens