Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C230A | the mutant shows about wild type activity but is less susceptible to oxidation than the wild type enzyme | Homo sapiens |
C277S | active-site mutant | Homo sapiens |
C370A | the kcat/Km is 33% of the wild type enzyme | Homo sapiens |
C371A | the kcat/Km is 5% of the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSSG | reversible inactivation, activity can be restored by treatment with dithiothreitol | Homo sapiens | |
N-acetyl-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 | active-site inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.2 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
11.5 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
30 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
30 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for catalytic activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is reversibly inactivated by oxidation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin-bound gamma-glutamine + 5-biotinamidopentylamine | - |
Homo sapiens | fibronectin N5-biotinamidopentylglutamine + NH3 | - |
? | |
Nalpha-benzyloxycarbonyl-L-Gln-Gly + alkylamine | - |
Homo sapiens | ? | - |
? | |
PQPQLPYPQPQLPY-NH2 + 5-biotinamidopentylamine | - |
Homo sapiens | ? | - |
? | |
QLQPFPQPQLPY + 5-biotinamidopentylamine | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TG2 | - |
Homo sapiens |
transglutaminase 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
0.19 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
0.0055 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
0.015 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens | |
0.017 | - |
Nalpha-benzyloxycarbonyl-L-Gln-Gly | wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C | Homo sapiens |