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Literature summary for 2.3.2.13 extracted from
- Activity of maize transglutaminase overexpressed in Escherichia coli inclusion bodies: an alternative to protein refolding (2011), Biotechnol. Prog., 27, 232-240.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in inclusion bodies of Escherichia coli BL21(DE3) cells | Zea mays |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity | Zea mays |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethylcasein + putrescine | Zea mays | - |
? | - |
? | |
| N-carbobenzoxy-L-glutaminylglycine + NH2OH | Zea mays | specific high-affinity substrate | L-glutamic acid-gamma-monohydroxamate + ? | - |
? | |
| thylakoid protein + putrescine | Zea mays | the incorporation of putrescine by the recombinant protein are 100fold greater using light-grown than dark-grown thylakoid protein extracts | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| using 55 mM MES, 264 mM NaCl, 11 mM KCl, 0.055% (w/v) PEG 3350, 550 mM guanidine-HCl, 2.2 mM MgCl2, 2.2 mM CaCl2, and 440 mM sucrose | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethylcasein + putrescine | - |
Zea mays | ? | - |
? | |
| N-carbobenzoxy-L-glutaminylglycine + NH2OH | specific high-affinity substrate | Zea mays | L-glutamic acid-gamma-monohydroxamate + ? | - |
? | |
| thylakoid protein + putrescine | the incorporation of putrescine by the recombinant protein are 100fold greater using light-grown than dark-grown thylakoid protein extracts | Zea mays | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamine:amine gamma-glutamyl-transferase | - |
Zea mays |
| TGase | - |
Zea mays |
| transglutaminase | - |
Zea mays |
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Release 2023.1 (January 2023)
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