Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is secreted as a pro-enzyme which is activated by the endoprotease TAMEP | Streptomyces mobaraensis |
Cloned (Comment) | Organism |
---|---|
expressed in Corynebacterum glutamicum strain ATTC13869 | Streptomyces mobaraensis |
Protein Variants | Comment | Organism |
---|---|---|
A10S | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
D14N | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
D3F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
D3L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
D3N | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
E28D | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
E58D | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
H289F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
H289Y | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
M16T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
M16T/G283S | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
N320D | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
N32D | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
N32D/E264D/N320T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
P12S | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Q74A | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Q74L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Q74N | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
R238F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
R238L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
R26F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
R26L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
R5K | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S199A | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S284T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S299L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S303A | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S303F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
S303T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
T77A | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
T77F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
T77L | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
T77S | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
V30D | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
V30I | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
V30T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
V65I | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
V6T | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
W59F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y34F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y34F/D268N | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y42H | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y75A | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y75F | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Y75H | the mutant shows higher specific activity compared to the wild type enzyme | Streptomyces mobaraensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Streptomyces mobaraensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | - |
- |
- |
Streptomyces mobaraensis s-8112 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Resource S column chromatography and Sephadex G25 gel filtration | Streptomyces mobaraensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.2 | - |
wild type enzyme, at pH 6.0 and 37°C | Streptomyces mobaraensis |
27.4 | - |
mutant enzyme N320D, at pH 6.0 and 37°C | Streptomyces mobaraensis |
28.5 | - |
mutant enzyme Y42H, at pH 6.0 and 37°C | Streptomyces mobaraensis |
28.6 | - |
mutant enzyme Y34F/D268N, at pH 6.0 and 37°C | Streptomyces mobaraensis |
28.7 | - |
mutant enzyme N32D/E264D/N320T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
28.9 | - |
mutant enzyme N32D, at pH 6.0 and 37°C | Streptomyces mobaraensis |
29.1 | - |
mutant enzyme V30D, at pH 6.0 and 37°C | Streptomyces mobaraensis |
29.7 | - |
mutant enzyme A10S, at pH 6.0 and 37°C | Streptomyces mobaraensis |
29.8 | - |
mutant enzyme M16T/G283S, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.1 | - |
mutant enzyme Q74L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.2 | - |
mutant enzyme V6T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.3 | - |
mutant enzyme R238F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.4 | - |
mutant enzyme R26L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.5 | - |
mutant enzyme D14N, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.5 | - |
mutant enzyme V30T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.6 | - |
mutant enzyme S303A, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.6 | - |
mutant enzyme S303F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.7 | - |
mutant enzyme R238L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
30.9 | - |
mutant enzyme S284T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
31.4 | - |
mutant enzyme T77L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
31.6 | - |
mutant enzyme Y75H, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.1 | - |
mutant enzyme S299L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.3 | - |
mutant enzyme E28D, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.3 | - |
mutant enzyme P12S, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.4 | - |
mutant enzyme D3L, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.4 | - |
mutant enzyme S303T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
32.7 | - |
mutant enzyme E58D, at pH 6.0 and 37°C | Streptomyces mobaraensis |
33 | - |
mutant enzyme H289Y, at pH 6.0 and 37°C | Streptomyces mobaraensis |
33.4 | - |
mutant enzyme R5K, at pH 6.0 and 37°C | Streptomyces mobaraensis |
33.5 | - |
mutant enzyme D3N, at pH 6.0 and 37°C | Streptomyces mobaraensis |
33.6 | - |
mutant enzyme T77A, at pH 6.0 and 37°C | Streptomyces mobaraensis |
33.8 | - |
mutant enzyme V30I, at pH 6.0 and 37°C | Streptomyces mobaraensis |
34.1 | - |
mutant enzyme T77F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
34.3 | - |
mutant enzyme Y34F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
34.4 | - |
mutant enzyme Q74N, at pH 6.0 and 37°C | Streptomyces mobaraensis |
34.9 | - |
mutant enzyme H289F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
35.5 | - |
mutant enzyme R26F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
35.5 | - |
mutant enzyme Y75A, at pH 6.0 and 37°C | Streptomyces mobaraensis |
35.8 | - |
mutant enzyme D3F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
35.9 | - |
mutant enzyme W59F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
36 | - |
mutant enzyme V65I, at pH 6.0 and 37°C | Streptomyces mobaraensis |
36.6 | - |
mutant enzyme T77S, at pH 6.0 and 37°C | Streptomyces mobaraensis |
37.4 | - |
mutant enzyme Q74A, at pH 6.0 and 37°C | Streptomyces mobaraensis |
41.7 | - |
mutant enzyme Y75F, at pH 6.0 and 37°C | Streptomyces mobaraensis |
42.5 | - |
mutant enzyme M16T, at pH 6.0 and 37°C | Streptomyces mobaraensis |
42.9 | - |
mutant enzyme S199A , at pH 6.0 and 37°C | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzoxy-L-glutaminylglycine + NH2OH | - |
Streptomyces mobaraensis | hydroxamic acid + ? | - |
? | |
N-carbobenzoxy-L-glutaminylglycine + NH2OH | - |
Streptomyces mobaraensis s-8112 | hydroxamic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTG | - |
Streptomyces mobaraensis |
protein-glutamine gamma-glutamyltransferase | - |
Streptomyces mobaraensis |
TGase | - |
Streptomyces mobaraensis |