Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 45% activity at 400 mM | Physarum polycephalum | |
Monodansylcadaverine | partial inhibition above 0.5 mM | Physarum polycephalum | |
N-ethylmaleimide | irreversible inhibition, inhibition increases with increasing Ca2+ concentrations, 50% inhibition at 1 mM Ca2+ | Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
Monodansylcadaverine | - |
Physarum polycephalum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement | Physarum polycephalum | |
Ca2+ | half-maximal activity at 0.7 mM, maximal activity at about 2 mM | Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
1 * 96000, gel filtration in the presence of 1 mM Ca2+ | Physarum polycephalum |
101000 | - |
gel filtration | Physarum polycephalum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | Physarum polycephalum | plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate | protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate, DEAE-cellulose, phenyl-Sepharose | Physarum polycephalum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.8 | - |
incorporation of monodansylcadaverine into N,N-dimethylcasein | Physarum polycephalum |
Storage Stability | Organism |
---|---|
-70°C, 6 months, little loss in activity | Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
monodansylcadaverine + N,N-dimethylcasein | - |
Physarum polycephalum | ? | - |
? | |
protein-bound gamma-glutamine + alkylamine | plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate | Physarum polycephalum | protein N5-alkylglutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 96000, gel filtration in the presence of 1 mM Ca2+ | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
less than 50% of maximal activity at pH 5,5 and pH 9.5 | Physarum polycephalum |