Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.13 extracted from

  • Mottahedeh, J.; Marsh, R.
    Characterization of 101-kDa transglutaminase from Physarum polycephalum and identification of LAV1-2 as substrate (1998), J. Biol. Chem., 273, 29888-29895.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
KCl 45% activity at 400 mM Physarum polycephalum
Monodansylcadaverine partial inhibition above 0.5 mM Physarum polycephalum
N-ethylmaleimide irreversible inhibition, inhibition increases with increasing Ca2+ concentrations, 50% inhibition at 1 mM Ca2+ Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
Monodansylcadaverine
-
Physarum polycephalum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Physarum polycephalum
Ca2+ half-maximal activity at 0.7 mM, maximal activity at about 2 mM Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
1 * 96000, gel filtration in the presence of 1 mM Ca2+ Physarum polycephalum
101000
-
gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein-bound gamma-glutamine + alkylamine Physarum polycephalum plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate protein N5-alkylglutamine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin sulfate, DEAE-cellulose, phenyl-Sepharose Physarum polycephalum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.8
-
incorporation of monodansylcadaverine into N,N-dimethylcasein Physarum polycephalum

Storage Stability

Storage Stability Organism
-70°C, 6 months, little loss in activity Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monodansylcadaverine + N,N-dimethylcasein
-
Physarum polycephalum ?
-
?
protein-bound gamma-glutamine + alkylamine plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate Physarum polycephalum protein N5-alkylglutamine + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 96000, gel filtration in the presence of 1 mM Ca2+ Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
less than 50% of maximal activity at pH 5,5 and pH 9.5 Physarum polycephalum