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Literature summary for 2.3.2.13 extracted from

  • Klein, J.D.; Guzman, E.; Kuehn, G.D.
    Purification and partial characterization of transglutaminase from Physarum polycephalum (1992), J. Bacteriol., 174, 2599-2605.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM; reversible Physarum polycephalum
EGTA 2 mM, irreversible Physarum polycephalum
N-ethylmaleimide 0.1 mM Physarum polycephalum
p-chloromercuribenzoate 0.1 mM Physarum polycephalum
putrescine 0.1-0.2 mM, substrate inhibition Physarum polycephalum
spermidine 0.1-0.2 mM, substrate inhibition Physarum polycephalum
spermine 0.1-0.2 mM, substrate inhibition Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0214
-
 spermidine
-
 Physarum polycephalum
0.0317
-
 spermine
-
 Physarum polycephalum
0.049
-
 putrescine
-
 Physarum polycephalum

Localization

Localization Comment Organism GeneOntology No. Textmining
spheroplast
-
 Physarum polycephalum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39600
-
 2 * 39600, SDS-PAGE Physarum polycephalum
77000
-
 gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein-bound gamma-glutamine + alkylamine Physarum polycephalum actin is probably the major endogenous substrate protein N5-alkylglutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
 slime mould, strain M3cV
-

Purification (Commentary)

Purification (Comment) Organism
15% polyethylene glycol, DEAE-cellulos, isoelectric focusing Physarum polycephalum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
-
 Physarum polycephalum

Storage Stability

Storage Stability Organism
-20°C, partially purified, 3 years Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protein-bound gamma-glutamine + alkylamine actin is probably the major endogenous substrate Physarum polycephalum protein N5-alkylglutamine + NH3
-
 ?
putrescine + bovine muscle actin preferred substrate Physarum polycephalum ?
-
 ?
putrescine + casein
-
 Physarum polycephalum ?
-
 ?
putrescine + N,N'-dimethylcasein spermine can replace putrescine Physarum polycephalum ?
-
 ?
putrescine + N,N'-dimethylcasein diaminopropane and cadaverine can replace putrescine Physarum polycephalum ?
-
 ?
putrescine + N,N'-dimethylcasein spermidine can replace putrescine Physarum polycephalum ?
-
 ?
spermidine + N,N'-dimethylcasein
-
 Physarum polycephalum ?
-
 ?
spermine + N,N'-dimethylcasein
-
 Physarum polycephalum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39600, SDS-PAGE Physarum polycephalum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.1 Physarum polycephalum