Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 85 mM sodium citrate, pH 5.6, 25.5% (w/v) polyethylene glycol 4000, 170 mM ammonium acetate, and 15% (v/v) glycerol | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
doripenem | - |
Mycobacterium tuberculosis | |
ertapenem | - |
Mycobacterium tuberculosis | |
Imipenem | - |
Mycobacterium tuberculosis | |
meropenem | - |
Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-tRNA1 + aminoacyl-tRNA2 | Mycobacterium tuberculosis | - |
tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? | |
peptidyl-tRNA1 + aminoacyl-tRNA2 | Mycobacterium tuberculosis CDC 1551 | - |
tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKV2 | - |
- |
Mycobacterium tuberculosis CDC 1551 | P9WKV2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) | Mycobacterium tuberculosis CDC 1551 | ? | - |
? | |
peptidyl-tRNA1 + aminoacyl-tRNA2 | - |
Mycobacterium tuberculosis | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? | |
peptidyl-tRNA1 + aminoacyl-tRNA2 | - |
Mycobacterium tuberculosis CDC 1551 | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LD-transpeptidase | - |
Mycobacterium tuberculosis |
LdtMt5 | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
malfunction | Mycobacterium tuberculosis lacking a functional copy of LdtMt5 displays aberrant growth and is more susceptible to killing by crystal violet, osmotic shock, and select carbapenem antibiotics | Mycobacterium tuberculosis |
physiological function | the enzyme LdtMt5 is necessary for properly maintaining cell wall integrity | Mycobacterium tuberculosis |