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Literature summary for 2.3.2.12 extracted from

  • Brammer Basta, L.A.; Ghosh, A.; Pan, Y.; Jakoncic, J.; Lloyd, E.P.; Townsend, C.A.; Lamichhane, G.; Bianchet, M.A.
    Loss of a functionally and structurally distinct LD-transpeptidase, LdtMt5, compromises cell wall integrity in Mycobacterium tuberculosis (2015), J. Biol. Chem., 290, 25670-25685.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 85 mM sodium citrate, pH 5.6, 25.5% (w/v) polyethylene glycol 4000, 170 mM ammonium acetate, and 15% (v/v) glycerol Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
doripenem
-
Mycobacterium tuberculosis
ertapenem
-
Mycobacterium tuberculosis
Imipenem
-
Mycobacterium tuberculosis
meropenem
-
Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2 Mycobacterium tuberculosis
-
tRNA1 + peptidyl(aminoacyl-tRNA2)
-
?
peptidyl-tRNA1 + aminoacyl-tRNA2 Mycobacterium tuberculosis CDC 1551
-
tRNA1 + peptidyl(aminoacyl-tRNA2)
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WKV2
-
-
Mycobacterium tuberculosis CDC 1551 P9WKV2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) Mycobacterium tuberculosis ?
-
?
additional information the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) Mycobacterium tuberculosis CDC 1551 ?
-
?
peptidyl-tRNA1 + aminoacyl-tRNA2
-
Mycobacterium tuberculosis tRNA1 + peptidyl(aminoacyl-tRNA2)
-
?
peptidyl-tRNA1 + aminoacyl-tRNA2
-
Mycobacterium tuberculosis CDC 1551 tRNA1 + peptidyl(aminoacyl-tRNA2)
-
?

Synonyms

Synonyms Comment Organism
LD-transpeptidase
-
Mycobacterium tuberculosis
LdtMt5
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction Mycobacterium tuberculosis lacking a functional copy of LdtMt5 displays aberrant growth and is more susceptible to killing by crystal violet, osmotic shock, and select carbapenem antibiotics Mycobacterium tuberculosis
physiological function the enzyme LdtMt5 is necessary for properly maintaining cell wall integrity Mycobacterium tuberculosis